Induction of apoptosis by Panduratin A isolated from Kaempferia pandurata in human colon cancer HT-29 cells.

Abstract

We previously showed that panduratin A isolated from an extract of Kaempferia pandurata (Zingiberaceae) was a strong inhibitor of cyclooxygenase-2 (COX-2) in RAW264.7 cells, suggesting a potential use of panduratin A as an anti-inflammatory agent. In the present study, we have investigated the effects of panduratin A on cytoplasmic levels of COX-2, as well as proliferation and apoptosis in human colon cancer cells HT-29. Cell proliferation and induction of apoptosis was determined by the MTT assay, DNA fragmentation measurement, flow cytometric analysis, nuclear staining and Western blotting. The MTT assay indicated that panduratin A exhibited cytotoxicity with an IC50 value of 28 microM. The cytotoxic effects of panduratin A were found to be accompanied by the dose-dependent induction of apoptosis as assessed by DNA fragmentation and apoptotic bodies. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in cleavage of poly(ADP-ribose) polymerase (PARP) with a concomitant decrease in procaspase-3 protein. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in human colon cancer cells, suggesting its potential use as a cancer chemopreventive and therapeutic agent.

• Authors: Jung-Mi Yun, Hoonjeong Kwon, Hasan Mukhtar, Jae-Kwan Hwang
• Journal: Planta medica (Planta Med) Vol. 71 Issue 6 Pg. 501-7 (Jun 2005) ISSN: 0032-0943 [Print] Germany
• PMID: 15971119 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Chalcones
• Cyclooxygenase Inhibitors
• Plant Extracts
• panduratin A

Topics

• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacology, therapeutic use)
• Apoptosis (drug effects)
• Chalcones
• Colonic Neoplasms (drug therapy, pathology)
• Cyclooxygenase Inhibitors (administration & dosage, pharmacology, therapeutic use)
• Flow Cytometry
• HT29 Cells (drug effects)
• Humans
• Phytotherapy
• Plant Extracts (administration & dosage, pharmacology, therapeutic use)
• Zingiberaceae