Abstract | OBJECTIVE: METHODS: Fibroblast cultures conditionally overexpressing MAGP-2 were developed. Cells were analyzed by Western blotting, Northern blotting, pulse-chase analysis, and immunofluorescence to assess the effect of MAGP-2 on type I collagen. RESULTS: CONCLUSION: This study shows that MAGP-2 stabilizes type I procollagen, identifying an important function of MAGP-2 in extracellular matrix homeostasis. It also suggests that MAGP-2 might mediate skin fibrosis in TSK mice and in patients with scleroderma.
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Authors | Raphael Lemaire, Joseph H Korn, J Michael Shipley, Robert Lafyatis |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 52
Issue 6
Pg. 1812-23
(Jun 2005)
ISSN: 0004-3591 [Print] United States |
PMID | 15934076
(Publication Type: Journal Article)
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Chemical References |
- Collagen Type I
- Contractile Proteins
- Extracellular Matrix Proteins
- RNA Splicing Factors
- microfibrillar protein
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Topics |
- Animals
- Cell Line
- Collagen Type I
(biosynthesis)
- Contractile Proteins
(metabolism)
- Extracellular Matrix Proteins
(metabolism)
- Fibroblasts
- Fibrosis
- Mice
- Microfibrils
(pathology)
- RNA Splicing Factors
- Scleroderma, Systemic
(metabolism, physiopathology)
- Skin
(pathology)
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