Determination of underivatised sterols and bile acid trimethyl silyl ether methyl esters by gas chromatography-mass spectrometry-single ion monitoring in faeces.

Abstract

A method for quantification of total faecal sterols and bile acids (BAs) in human stool by using gas chromatography-mass spectrometry-single ion monitoring (GC-MS-SIM) is described. Cholesterol, coprostanol, coprostanone, cholestanol, iso-lithocholic acid (iso-LCA), lithocholic acid (LCA), iso-deoxycholic acid (iso-DCA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), cholic acid (CA), and 12-oxo-deoxycholic acid (12-oxo-DCA) in faeces of 86 healthy subjects were determined. The sample preparation for sterol analysis requires hydrolysis and liquid extraction from matrix, but no derivatisation. The GC-flame ionisation detection (FID) and total ion current (TIC) in GC-MS were not sufficient for sterol and BA determination, whereas selectivity and specificity of the GC-MS-SIM ensured the analysis of sterols and BAs in faeces.

• Authors: Sylvia Keller, Gerhard Jahreis
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 813 Issue 1-2 Pg. 199-207 (Dec 25 2004) ISSN: 1570-0232 [Print] Netherlands
• PMID: 15556534 (Publication Type: Journal Article)

Chemical References

• Bile Acids and Salts
• Esters
• Sterols

Topics

• Bile Acids and Salts (analysis, chemistry)
• Calibration
• Esters (analysis)
• Feces (chemistry)
• Gas Chromatography-Mass Spectrometry (methods)
• Reproducibility of Results
• Sterols (analysis)