Effects on rat thalamic proteome by acute and subchronic MK-801-treatment.

Abstract

Since the symptoms of intoxication with non-competitive N-methyl-D-aspartate (NMDA) receptor antagonists closely mimic symptoms in patients with schizophrenia, [+]-5-methyl-10,11-dihydro-5H-dibenzo-[a,d]-cycloheptene-5,10-iminehydrogenmaleate (MK-801)-treated rodents are often used as a model for schizophrenia. In most studies, acute injections of MK-801 to rats have been used, but in some studies, longer periods of treatment have been performed. In our previous work, alterations in mRNA/protein expression were screened in the cerebral cortex of MK-801 treated rats. Different proteins were altered in different treatment courses of MK-801. The main objective of the present study was to evaluate different treatment periods of treatment with MK-801 in rats as a model for schizophrenia. Thalamus proteins from treated (acute, six and 12 days) and control rats were analyzed with two-dimensional gel electrophoresis and mass spectrometry. Our results show that different treatment times of MK-801 to rats give different biochemical results. Therefore, it is important to use the same treatment time in studies that will be compared.

• Authors: Linda Paulson, Peter Martin, Elisabeth Ljung, Kaj Blennow, Pia Davidsson
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 505 Issue 1-3 Pg. 103-9 (Nov 28 2004) ISSN: 0014-2999 [Print] Netherlands
• PMID: 15556142 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteome
• Dizocilpine Maleate

Topics

• Animals
• Dizocilpine Maleate (pharmacology)
• Dose-Response Relationship, Drug
• Electrophoresis, Gel, Two-Dimensional
• Male
• Proteome (analysis)
• Rats
• Rats, Sprague-Dawley
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Thalamus (drug effects, metabolism)
• Time Factors