[Nobel genes improve accuracy in detection of peritoneal micro-metastasis of gastric cancer to decide indication for chemotherapy].

Abstract

Peritoneal metastasis is the most frequent form of recurrence for advanced gastric cancer. We previously performed a global analysis of the gene expression of gastric cancer cell lines established from peritoneal metastasis with cDNA microarray. One of the up-regulated genes is L-3 phosphoserine phosphatase (L3-PP). We have examined its potential as a novel marker for the detection of peritoneal micrometastasis of gastric cancer. L3-PP mRNA in peritoneal wash in 93 gastric cancer patients was quantified for comparison of carcinoembryonic antigen (CEA) mRNA by means of real-time RT-PCR to predict peritoneal recurrence. The quantity of L3-PP and CEA correlated with wall penetration. Eleven out of 18 cases with peritoneal dissemination were L3-PP+ (61% sensitivity). For three out of 18 cases of peritoneal dissemination, only L3-PP could detect micrometastasis of gastric cancer. Consequently, free cancer cells that cannot be detected by CEA mRNA could be detected using L3-PP mRNA. Although CEA alone was not sufficient, L3-PP and CEA in combination can attain a higher accuracy of detection.

• Authors: Katsumi Shimomura, Masaharu Ohgaki, Naoki Kakihara, Hiroomi Matsumura, Keigo Miyata, Ryouji Iizuka, Wataru Ikawa, Masataka Shimotsuma, Atsushi Takenaka, Chouhei Sakakura, Akeo Hagiwara, Hisakazu Yamagishi
• Journal: Gan to kagaku ryoho. Cancer & chemotherapy (Gan To Kagaku Ryoho) Vol. 31 Issue 11 Pg. 1912-4 (Oct 2004) ISSN: 0385-0684 [Print] Japan
• PMID: 15553757 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Biomarkers, Tumor
• Carcinoembryonic Antigen
• Genetic Markers
• Phosphoric Monoester Hydrolases
• phosphoserine phosphatase

Topics

• Aged
• Aged, 80 and over
• Biomarkers, Tumor (analysis)
• Carcinoembryonic Antigen (analysis)
• Cell Line, Tumor
• Female
• Gene Expression
• Genetic Markers (genetics)
• Humans
• Male
• Middle Aged
• Peritoneal Neoplasms (diagnosis, drug therapy, secondary)
• Phosphoric Monoester Hydrolases (analysis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Sensitivity and Specificity
• Stomach Neoplasms (pathology)