Abstract | PURPOSE: METHODS: Mice were immunized with MOG35-55 or MOG40-54 peptides emulsified in complete Freund's adjuvant (CFA). Pertussis toxin (PTX) was injected intraperitoneally 1 day before and after immunization. For disease induction by adoptive transfer of primed cells, donor C57BL/6 mice were received with T-cell blasts (1-6 x 10(6)/mouse). Both EAE and ON were observed by either clinical signs or histology. RESULTS: ON developed in a high proportion of B6 mice treated with either protocol. The most severe inflammation was observed in the adoptively transferred mice. The induced ON was most frequently bilateral. In either actively or adoptively transferred diseases, both association and dissociation of EAE and ON was observed. CONCLUSIONS: Different MOG-specific T-cell subsets might be involved in the pathogenesis of EAE and ON. A better understanding of the pathogenesis of ON after induction by MOG may have important diagnostic and therapeutic implications.
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Authors | Hui Shao, Zhigang Huang, Sheher L Sun, Henry J Kaplan, Deming Sun |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 45
Issue 11
Pg. 4060-5
(Nov 2004)
ISSN: 0146-0404 [Print] United States |
PMID | 15505056
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Glycoproteins
- Mog protein, mouse
- Myelin Proteins
- Myelin-Associated Glycoprotein
- Myelin-Oligodendrocyte Glycoprotein
- Peptide Fragments
- myelin oligodendrocyte glycoprotein (35-55)
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Topics |
- Adoptive Transfer
- Animals
- Encephalomyelitis, Autoimmune, Experimental
(etiology, pathology)
- Glycoproteins
(immunology)
- Immunization
(adverse effects)
- Injections, Intraperitoneal
- Mice
- Mice, Inbred C57BL
- Myelin Proteins
- Myelin-Associated Glycoprotein
(immunology)
- Myelin-Oligodendrocyte Glycoprotein
- Optic Neuritis
(etiology, pathology)
- Peptide Fragments
(immunology)
- T-Lymphocytes
(immunology)
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