Induction of apoptosis in human promyelocytic leukemia HL-60 cells by Ampelopsis cantoniensis crude extract.

Abstract

The crude extract of Ampelopsis cantoniensis induced apoptosis in human promyelocytic leukemia HL-60 cells and this induction was investigated by flow cytometric analysis, DNA gel electrophoresis and poly (ADP-ribose) fluorescence staining. The results demonstrated that this extract induced dose-dependent cytotoxicity and apoptosis. The level of active caspase-3 was increased after treatment with the crude extract for 24 hours.

• Authors: Tzu-Wei Tan, Huei-Yann Tsai, Yuh-Fung Chen, Jing-Gung Chung
• Journal: In vivo (Athens, Greece) (In Vivo) 2004 Jul-Aug Vol. 18 Issue 4 Pg. 457-62 ISSN: 0258-851X [Print] Greece
• PMID: 15369184 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• DNA, Neoplasm
• Drugs, Chinese Herbal
• RNA, Neoplasm
• CASP3 protein, human
• Caspase 3
• Caspases

Topics

• Ampelopsis (chemistry)
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Caspase 3
• Caspases (genetics, metabolism)
• Cell Survival (drug effects)
• DNA Fragmentation
• DNA, Neoplasm (analysis)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology)
• Flow Cytometry
• HL-60 Cells (drug effects, pathology)
• Humans
• Leukemia, Promyelocytic, Acute (drug therapy, pathology)
• RNA, Neoplasm (analysis)
• Reverse Transcriptase Polymerase Chain Reaction