Crystal structure of the carboxyltransferase domain of acetyl-coenzyme A carboxylase in complex with CP-640186.

Abstract

Acetyl-coenzyme A carboxylases (ACCs) are important targets for the development of therapeutic agents against obesity, diabetes, and other diseases. CP-640186 is a potent inhibitor of mammalian ACCs and can reduce body weight and improve insulin sensitivity in test animals. It is believed to target the carboxyltransferase (CT) domain of these enzymes. Here we report the crystal structure of the yeast CT domain in complex with CP-640186. The inhibitor is bound in the active site at the interface of a dimer of the CT domain. CP-640186 has tight interactions with the putative biotin binding site in the CT domain and demonstrates a distinct mode of inhibiting the CT activity as compared to the herbicides that inhibit plant ACCs. The affinity of inhibitors for the CT domain has been assessed using kinetic and fluorescence anisotropy binding studies. The structural information identifies three regions for drug binding in the active site of CT.

• Authors: Hailong Zhang, Benjamin Tweel, Jiang Li, Liang Tong
• Journal: Structure (London, England : 1993) (Structure) Vol. 12 Issue 9 Pg. 1683-91 (Sep 2004) ISSN: 0969-2126 [Print] United States
• PMID: 15341732 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• 3-(2-methoxy-5-trifluoromethoxybenzyl)amino-1-methyl-2-phenylpiperidine
• CP-640186
• Isoenzymes
• Morpholines
• Piperidines
• Saccharomyces cerevisiae Proteins
• Acetyl-CoA Carboxylase

Topics

• Acetyl-CoA Carboxylase (antagonists & inhibitors, chemistry, metabolism)
• Amino Acid Sequence
• Animals
• Binding Sites
• Crystallography, X-Ray
• Humans
• Isoenzymes (chemistry, metabolism)
• Models, Molecular
• Molecular Sequence Data
• Molecular Structure
• Morpholines (chemistry, metabolism)
• Piperidines (chemistry, metabolism)
• Protein Binding
• Protein Structure, Tertiary
• Saccharomyces cerevisiae Proteins (chemistry, genetics, metabolism)
• Sequence Alignment