Abstract | PURPOSE: EXPERIMENTAL DESIGN: Five melanoma cell lines were analyzed by reverse transcription-PCR/Southern blotting for preproTRH message. The same melanoma lines and two melanocyte lines were examined by immunocytochemistry for TRH protein expression and for growth response to exogenous TRH. Immunohistochemistry was used to test for TRH protein in sections of 19 melanomas, 33 dysplastic nevi, and 27 benign nevi. RESULTS: TRH message and protein were detected in all melanoma cell lines examined. Melanocytes were also found to express TRH protein. Four of the five melanoma cell lines but neither melanocyte line responded with a increase in proliferation to low concentrations of exogenous TRH. TRH immunoreactivity was observed in 12 of 19 melanomas (63%), 23 of 33 (69.7%) dysplastic nevi, and 14 of 27 (51.9%) benign nevi. Expression in dysplastic nevi was significantly greater than in benign nevi. Upon separate analysis of nevi from melanoma patients, the difference between dysplastic and benign nevi was even more significant. However, in healthy individuals, no difference between dysplastic and benign nevi was observed. Furthermore, dysplastic nevi from melanoma patients had a significantly higher percentage of TRH-positive cells when compared with healthy individuals. CONCLUSIONS:
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Authors | Julie A Ellerhorst, Aresu A Naderi, Marilyn K Johnson, Pauline Pelletier, Victor G Prieto, A Hafeez Diwan, Marcella M Johnson, Debra C Gunn, Sandra Yekell, Elizabeth A Grimm |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 10
Issue 16
Pg. 5531-6
(Aug 15 2004)
ISSN: 1078-0432 [Print] United States |
PMID | 15328193
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA Primers
- Thyrotropin-Releasing Hormone
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Topics |
- Cell Line, Tumor
- DNA Primers
- Gene Expression Regulation, Neoplastic
- Humans
- Melanoma
(genetics, pathology)
- Nevus
(genetics, pathology)
- Predictive Value of Tests
- Reverse Transcriptase Polymerase Chain Reaction
- Skin Neoplasms
(genetics, pathology)
- Thyrotropin-Releasing Hormone
(analysis, genetics)
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