Over a decade of experience with preimplantation genetic diagnosis: a multicenter report.

Abstract

OBJECTIVE:

To review a 12-year experience of the world's three largest preimplantation genetic diagnosis (PGD) centers.

DESIGN:

Multicenter analysis of the clinical outcome of PGD.

SETTING:

In vitro fertilization programs at the Reproductive Genetics Institute, Chicago, Illinois; Saint Barnabas Medical Center, West Orange, New Jersey; and SISMER, Bologna, Italy.

PATIENT(S):

Poor-prognosis IVF patients, patients carrying balanced chromosomal translocations, and couples at risk for producing children with Mendelian disorders.

INTERVENTION(S):

In vitro fertilization, intracytoplasmic sperm injection, polar body removal, blastomere biopsy, and ET.

MAIN OUTCOME MEASURE(S):

DNA or chromosomal analysis of biopsied polar bodies or blastomeres, implantation and clinical pregnancy rates, and live-born pregnancy outcome.

RESULT(S):

A total of 754 babies have been born as a result of 4,748 PGD attempts, which shows the expanded application and the practical relevance of PGD for single-gene disorders, chromosomal aneuploidies and translocations, late-onset diseases with genetic predisposition, and nondisease testing in couples at need for human leukocyte antigens-matched offspring for treatment of affected siblings.

CONCLUSION(S):

Preimplantation genetic diagnosis is evolving to become a clinical option for couples at risk for producing offspring with Mendelian diseases, has a positive numerical impact in standard assisted reproduction practices through aneuploidy testing, and reduces by at least fourfold the spontaneous abortion rate in couples carrying translocations.

• Authors: Yury Verlinsky, Jacques Cohen, Santiago Munne, Luca Gianaroli, Joe Leigh Simpson, Anna Pia Ferraretti, Anver Kuliev
• Journal: Fertility and sterility (Fertil Steril) Vol. 82 Issue 2 Pg. 292-4 (Aug 2004) ISSN: 0015-0282 [Print] United States
• PMID: 15302270 (Publication Type: Journal Article)

Topics

• Blastula
• Female
• Genetic Diseases, Inborn (genetics, prevention & control)
• Genetic Testing (trends)
• Humans
• Pregnancy
• Pregnancy Outcome