Abstract | BACKGROUND: MATERIALS AND METHODS:
Warm ischemia was sustained for 4 hours. The animals were divided into 4 groups: (1) sham group, (2) control group (saline injection), (3) MCI group (MCI-186 injection), and (4) EPC group ( EPC-K1 [( l-ascorbic acid 2-[3,4-dihydro-2, 5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1- benzopyran-6-yl hydrogen phosphate] potassium salt], a hydroxyl-radical scavenger, injection). Wet and dry (W/D) weights of the gastrocnemius and tibialis anterior muscles, muscle contractile function, and serum levels of creatine phosphokinase (CPK), lactate dehydrogenase (LDH), glutamic oxaloacetic transminase (GOT), and mitochondrial glutamic oxaloacetic transminase (GOT-m) were measured after 24 h of reperfusion. The cytotoxic aldehydes malondialdehyde and 4-hydroxy-2-nonenal as indices of lipid peroxidation (LPO), and neutrophil-specific enzyme myeloperoxidase (MPO) as an index of neutrophil infiltration, were measured in the gastrocnemius muscle. RESULTS: Contractile functions in the MCI and EPC groups were significantly better than in the control group. In the tibialis anterior muscle, the contractile function was better in the MCI group than in the EPC group. W/D ratios and serum levels of CPK, LDH, GOT, and GOT-m were lower in the sham and MCI groups than in the control group. Levels of LPO and MPO activity were significantly lower in the MCI and EPC groups than in the control group. Histological findings demonstrated that inflammatory tissue reactions rarely occurred in the MCI group. CONCLUSION:
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Authors | H Irie, T Kato, K Ikebe, T Tsuchida, Y Oniki, K Takagi |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 120
Issue 2
Pg. 312-9
(Aug 2004)
ISSN: 0022-4804 [Print] United States |
PMID | 15234228
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Enzymes
- Free Radical Scavengers
- Peroxidase
- Edaravone
- Antipyrine
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Topics |
- Amputation, Surgical
- Animals
- Antioxidants
(pharmacology)
- Antipyrine
(analogs & derivatives, pharmacology)
- Body Water
(metabolism)
- Edaravone
- Enzymes
(blood)
- Free Radical Scavengers
(pharmacology)
- Hindlimb
(surgery)
- Lipid Peroxidation
- Male
- Muscle, Skeletal
(blood supply, pathology, physiopathology)
- Peroxidase
(metabolism)
- Rats
- Rats, Inbred Lew
- Reperfusion Injury
(metabolism, pathology, physiopathology)
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