Abstract | BACKGROUND: Some idiopathic interstitial pneumonias (IIPs) are characterised by fibroproliferation and deposition of extracellular matrix. Because efficacious treatment options are limited, research has been directed towards understanding the cytokine networks that may affect fibroblast activation and, hence, the progression of certain IIPs. AIMS: To examine the expression of interleukin 4 (IL-4), IL-13, and their corresponding receptor subunits in the various forms of IIP and normal patient groups. METHODS: RESULTS: Molecular analysis demonstrated that IL-13Ralpha2, IL-13Ralpha1, and IL-4Ralpha were present in a greater proportion of upper and lower lobe biopsies from patients with UIP than patients with NSIP and RBILD. Immunohistochemical analysis of patients with UIP, NSIP, and RBILD revealed interstitial staining for all three receptor subunits, whereas such staining was only seen in mononuclear cells present in normal SLBs. Fibroblastic foci in patients with UIP strongly stained for IL-4Ralpha and IL-13Ralpha2. Localised expression of IL-4Ralpha was also seen in SLBs from patients with NSIP but not in other groups. CONCLUSION: Some histological subtypes of IIP are associated with increased pulmonary expression of receptor subunits responsive to IL-4 and IL-13. These findings may be of particular importance in understanding the pathogenesis of IIP and, more importantly, may provide important novel therapeutic targets.
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Authors | C Jakubzick, E S Choi, S L Kunkel, H Evanoff, F J Martinez, R K Puri, K R Flaherty, G B Toews, T V Colby, E A Kazerooni, B H Gross, W D Travis, C M Hogaboam |
Journal | Journal of clinical pathology
(J Clin Pathol)
Vol. 57
Issue 5
Pg. 477-86
(May 2004)
ISSN: 0021-9746 [Print] England |
PMID | 15113854
(Publication Type: Journal Article)
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Chemical References |
- IL13RA1 protein, human
- Interleukin-13
- Interleukin-13 Receptor alpha1 Subunit
- RNA, Messenger
- Receptors, Interleukin
- Receptors, Interleukin-13
- Receptors, Interleukin-4
- Interleukin-4
- Interferon-gamma
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Topics |
- Adult
- Aged
- Biopsy
- Female
- Gene Expression
- Humans
- Interferon-gamma
(genetics, metabolism)
- Interleukin-13
(genetics, metabolism)
- Interleukin-13 Receptor alpha1 Subunit
- Interleukin-4
(genetics, metabolism)
- Lung
(metabolism)
- Lung Diseases, Interstitial
(metabolism, pathology)
- Male
- Middle Aged
- Polymerase Chain Reaction
(methods)
- RNA, Messenger
(genetics)
- Receptors, Interleukin
(genetics, metabolism)
- Receptors, Interleukin-13
- Receptors, Interleukin-4
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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