Local tissue hypoxia and formation of nasal polyps.

Abstract

OBJECTIVE:

To explore the response of nasal mucosa epithelial cells to hypoxia in terms of formation of nasal polyps (NP).

METHODS:

Epithelial cells of NP and inferior turbinate (IT) were cultured serum-free under normal oxygen and hypoxic circumstances with stimulation of IL-1 beta and TNF alpha. The vascular endothelial growth factor (VEGF) mRNA and VEGF protein levels of the cultured cells were detected using in situ hybridization and ELISA, respectively.

RESULTS:

The expression of VEGF mRNA was significantly higher in epithelial cells of NP than in IT exposed to pro-inflammatory cytokines or hypoxia (P < 0.01). VEGF levels were higher in NP epithelial cells than those of IT (P < 0.01) under hypoxia.

CONCLUSION:

VEGF-induced by hypoxia is very important for the early stages of forming polyps.

• Authors: Shu Jiang, Zhen Dong, Dongdong Zhu, Zhanquan Yang
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 116 Issue 2 Pg. 243-7 (Feb 2003) ISSN: 0366-6999 [Print] China
• PMID: 12775240 (Publication Type: Journal Article)

Chemical References

• Endothelial Growth Factors
• Intercellular Signaling Peptides and Proteins
• Interleukin-1
• Lymphokines
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors
• Erythropoietin

Topics

• Cell Hypoxia (physiology)
• Cells, Cultured
• Endothelial Growth Factors (genetics)
• Enzyme-Linked Immunosorbent Assay
• Erythropoietin (genetics)
• Humans
• Intercellular Signaling Peptides and Proteins (genetics)
• Interleukin-1 (pharmacology)
• Lymphokines (genetics)
• Nasal Mucosa (metabolism)
• Nasal Polyps (etiology, metabolism)
• RNA, Messenger (analysis)
• Tumor Necrosis Factor-alpha (pharmacology)
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors