Abstract | AIM: METHODS:
Hepatic cirrhosis was induced in Sprague-Dawley rats by administration of thioacetamide intraperitoneally for 9-12 weeks. Collagenase IV was perfused in situ for isolation of hepatocytes. The expression of GHR and its mRNA in cirrhotic livers was studied with radio- ligand binding assay, RT-PCR and digital image analysis. RESULTS: One class of specific growth hormone-binding site, GHR, was detected in hepatocytes and hepatic tissue of cirrhotic livers. The binding capacity of GHR (R(T), fmol/mg protein) in rat cirrhotic liver tissue (30.8+/-1.9) was significantly lower than that in normal control (74.9+/-3.9) at the time point of the ninth week after initiation of induction of cirrhosis (n=10, P<0.05), and it decreased gradually along with the accumulation of collagen in the process of formation and development of liver cirrhosis (P<0.05). The number of binding sites (X10(4)/cell) of GHR on rat cirrhotic hepatocytes (0.86+/-0.16) was significantly lower than that (1.28+/-0.24) in control (n=10, P<0.05). The binding affinity of GHR among liver tissue, hepatocytes of various groups had no significant difference (P>0.05). The expression of GHR mRNA (riOD, pixel) in rat cirrhotic hepatic tissues (23.3+/-3.1) was also significantly lower than that (29.3+/-3.4) in normal control (n=10, P<0.05). CONCLUSION:
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Authors | Hong-Tao Wang, Shuang Chen, Jie Wang, Qing-Jia Ou, Chao Liu, Shu-Sen Zheng, Mei-Hai Deng, Xiao-Ping Liu |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 9
Issue 4
Pg. 765-70
(Apr 2003)
ISSN: 1007-9327 [Print] United States |
PMID | 12679928
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acetamides
- RNA, Messenger
- Receptors, Somatotropin
- acetamide
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Topics |
- Acetamides
- Animals
- Gene Expression Regulation
- Liver Cirrhosis, Experimental
(chemically induced, genetics, physiopathology)
- RNA, Messenger
(genetics)
- Rats
- Rats, Sprague-Dawley
- Receptors, Somatotropin
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
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