Expression levels of FUT6 gene transfected into human colon carcinoma cells switch two sialyl-Lewis X-related carbohydrate antigens with distinct properties in cell adhesion.

Abstract	A human colon carcinoma cell line KM12-LX, expressing low levels of monoclonal antibody (mAb) FH6 epitope, was transfected with alpha 1,3-fucosyltransferase VI cDNA. Clonal populations with high or intermediate expression levels of the mRNA, shown by RT-PCR (FT6hi and FT6in cells, respectively) were obtained. FT6hi cells were found to express both mAb FH6 and KM93 epitopes by flow-cytometric analysis, whereas FT6in cells expressed mAb FH6 epitopes but not mAb KM93 epitopes. The mAb FH6-binding was abrogated by endo-beta-galactosidase treatment of FT6in, but not FT6hi, cells. FT6hi but not FT6in cells adhered to Chinese-hamster-ovary cells expressing human E-selectin. FT6in cells adhered to sections of mouse liver and the adhesion was blocked by treatment of the cells with endo-beta-galactosidase. The results indicate that endo-beta-galactosidase-sensitive and mAb FH6-reactive carbohydrate chains are generated under the control of expression levels of FUT6 and involved in the adhesion of colon carcinoma cells to liver sections.
Authors	Akira Kanoh, Masayuki Ota, Hisashi Narimatsu, Tatsuro Irimura
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 303 Issue 3 Pg. 896-901 (Apr 11 2003) ISSN: 0006-291X [Print] United States
PMID	12670495 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antibodies, Monoclonal E-Selectin Oligosaccharides RNA, Messenger RNA, Neoplasm Sialyl Lewis X Antigen Fucosyltransferases galactoside 3-fucosyltransferase Glycoside Hydrolases keratan-sulfate endo-1,4-beta-galactosidase beta-Galactosidase
Topics	Animals Antibodies, Monoclonal CHO Cells Cell Adhesion Colonic Neoplasms (enzymology, genetics, immunology, pathology) Cricetinae E-Selectin (genetics, metabolism) Fucosyltransferases (genetics) Gene Expression Glycoside Hydrolases Humans In Vitro Techniques Liver (metabolism, pathology) Mice Oligosaccharides (metabolism) RNA, Messenger (genetics, metabolism) RNA, Neoplasm (genetics, metabolism) Sialyl Lewis X Antigen Transfection Tumor Cells, Cultured beta-Galactosidase (metabolism)

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