Proliferative activity of bile from congenital choledochal cyst patients.

Abstract

AIM:

To explore the potential carcinogenicity of bile from congenital choledochal cyst (CCC) patients and the mechanism of the carcinogenesis in congenital choledochal cyst patients.

METHODS:

20 bile samples from congenital choledochal cyst patients and 10 normal control bile samples were used for this study. The proliferative effect of bile was measured by using Methabenzthiazuron (MTT) assay; Cell cycle and apoptosis were analyzed by using flow cytometry (FCM), and the PGE(2) levels in the supernatant of cultured cholangiocarcinoma cells were quantitated by enzyme-linked immunoabsorbent assay (ELISA).

RESULTS:

CCC bile could significantly promote the proliferation of human cholangiocarcinoma QBC939 cells compared with normal bile (P=0.001) and negative control group (P=0.002), and the proliferative effect of CCC bile could be abolished by addition of cyclooxygenase-2 specific inhibitor celecoxib (20 microM). The QBC939 cells proliferative index was increased significantly after treated with 1 % bile from CCC patient (P=0.008) for 24 h, the percentage of S phase (29.48+/-3.27)% was increased remarkably (P<0.001) compared with normal bile (11.72+/-2.70) %, and the percentage of G0/G1 phase (54.19+/-9.46) % was decreased remarkably (P=0.042) compared with normal bile (69.16+/-10.88) %, however, bile from CCC patient had no significant influence on apoptosis of QBC939 cells (P=0.719).

CONCLUSION:

Bile from congenital choledochal cyst patients can promote the proliferation of human cholangiocarcinoma QBC939 cells via COX-2 and PGE(2) pathway.

• Authors: Gao-Song Wu, Sheng-Quan Zou, Xian-Wen Luo, Jian-Hong Wu, Zheng-Ren Liu
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 9 Issue 1 Pg. 184-7 (Jan 2003) ISSN: 1007-9327 [Print] United States
• PMID: 12508379 (Publication Type: Journal Article)

Chemical References

• Carcinogens
• Cyclooxygenase 2 Inhibitors
• Cyclooxygenase Inhibitors
• Isoenzymes
• Membrane Proteins
• Pyrazoles
• Sulfonamides
• Cyclooxygenase 2
• PTGS2 protein, human
• Prostaglandin-Endoperoxide Synthases
• Celecoxib
• Dinoprostone

Topics

• Adolescent
• Adult
• Animals
• Apoptosis
• Bile (metabolism)
• Carcinogens
• Celecoxib
• Cell Cycle
• Cell Division
• Child
• Child, Preschool
• Choledochal Cyst (metabolism)
• Cyclooxygenase 2
• Cyclooxygenase 2 Inhibitors
• Cyclooxygenase Inhibitors (metabolism)
• Dinoprostone (metabolism)
• Female
• Flow Cytometry
• Humans
• Isoenzymes (metabolism)
• Male
• Membrane Proteins
• Middle Aged
• Prostaglandin-Endoperoxide Synthases (metabolism)
• Pyrazoles
• Sulfonamides (metabolism)
• Tumor Cells, Cultured