Nitric oxide-mediated Ca2+/calmodulin-dependent protein kinase IV activity during hypoxia in neuronal nuclei from newborn piglets.

Abstract

The present study tested the hypothesis that hypoxia results in increased Ca(2+)/calmodulin-dependent protein kinase IV (CaM kinase IV) activity and that inhibition of nitric oxide (NO) synthase by N-nitro-L-arginine (NNLA) prevents the hypoxia- induced increase in neuronal nuclear CaM kinase IV activity in newborn piglets. CaM kinase IV activity was determined in normoxic (Nx), hypoxic (Hx), and NNLA-pretreated Hx piglets. Cerebral hypoxia was confirmed biochemically. There was a significant difference between CaM kinase IV activity (pmoles/mg protein/min) in Nx (285.22+/-86.12), Hx (494.77+/-99.79, P<0.05 vs. Nx), and NNLA-pretreated Hx (249.55+/-53.85)(P=NS vs. Nx, P<0.05 vs. Hx) animals. The results demonstrate that the cerebral tissue hypoxia results in an increase in neuronal nuclear CaM kinase IV activity, and the hypoxia-induced increase in CaM kinase IV activity is NO-mediated.

• Authors: Alan B Zubrow, Maria Delivoria-Papadopoulos, Qazi M Ashraf, Karen I Fritz, Om P Mishra
• Journal: Neuroscience letters (Neurosci Lett) Vol. 335 Issue 1 Pg. 5-8 (Dec 19 2002) ISSN: 0304-3940 [Print] Ireland
• PMID: 12457729 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Calcium Compounds
• Enzyme Inhibitors
• Nitroarginine
• Nitric Oxide
• Nitric Oxide Synthase
• Calcium-Calmodulin-Dependent Protein Kinase Type 4
• Calcium-Calmodulin-Dependent Protein Kinases

Topics

• Animals
• Animals, Newborn
• Brain (drug effects, enzymology, metabolism)
• Calcium Compounds (metabolism)
• Calcium-Calmodulin-Dependent Protein Kinase Type 4
• Calcium-Calmodulin-Dependent Protein Kinases (drug effects, metabolism)
• Cell Nucleus (drug effects, enzymology, metabolism)
• Enzyme Inhibitors (pharmacology)
• Hypoxia, Brain (enzymology, metabolism)
• Neurons (drug effects, enzymology, metabolism)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase (antagonists & inhibitors, metabolism)
• Nitroarginine (pharmacology)
• Swine