In vitro effects of Habu snake venom on cultured mesangial cells.

Abstract

BACKGROUND:

Habu snake venom (HSV)-induced glomerulonephritis is a unique model showing a progressive course of mesangial proliferation. To elucidate the in vitro effects of HSV, we examined whether HSV itself could have direct effects on the cultured mesangial cells, such as cell proliferation and activation of chemokine gene expression.

METHODS:

The incorporation of 5-[(125)I]iodo-2'-deoxyuridine was measured with a gamma-counter, and gene expressions of growth factors, chemokines and cytokines were evaluated by a real time quantitative PCR.

RESULTS:

We demonstrated that excessive or continuous HSV stimulation decreased a mesangial cell viability. However, adequate and temporary HSV stimulation induced proliferation of mesangial cells in vitro along with a significant elevation of monocyte chemoattractant protein-1 (MCP-1) mRNA levels. In addition to these in vitro results, we showed that MCP-1 mRNA levels increased in renal cortices of glomerulonephritis induced by HSV. Immunohistochemistry also showed a positive staining for MCP-1 in the marginal area of glomerulus with mesangiolysis.

CONCLUSIONS:

These data suggest that HSV itself may elicit direct biological effects on mesangial cells which may participate in pathophysiology of glomerulonephritis induced by HSV.

• Authors: Atsushi Kubo, Masayuki Iwano, Yoshiyuki Kobayashi, Yusuke Kyoda, Yoshitaka Isumi, Naoki Maruyama, Kenichi Samejima, Yoshiko Dohi, Naoto Minamino, Kunio Yonemasu
• Journal: Nephron (Nephron) Vol. 92 Issue 3 Pg. 665-72 ( 2002) ISSN: 1660-8151 [Print] Switzerland
• PMID: 12372952 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2002 S. Karger AG, Basel

Chemical References

• Chemokine CCL2
• Crotalid Venoms
• Growth Substances
• Interleukin-1
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• DNA

Topics

• Animals
• Cell Division (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Chemokine CCL2 (analysis, genetics)
• Crotalid Venoms (pharmacology)
• DNA (biosynthesis)
• Gene Expression (drug effects)
• Glomerular Mesangium (chemistry, cytology, drug effects)
• Glomerulonephritis, Membranoproliferative (chemically induced, pathology, physiopathology)
• Growth Substances (genetics)
• Immunohistochemistry
• In Vitro Techniques
• Interleukin-1 (genetics)
• Macrophages (cytology, drug effects)
• Male
• RNA, Messenger (analysis)
• Rats
• Rats, Wistar
• Trimeresurus
• Tumor Necrosis Factor-alpha (genetics)