In vivo electroporation is an efficient means of increasing plasmid
DNA delivery to normal tissues, such as skin and muscle, as well as directly to
tumors. In the experiments described here, plasmid
DNA was delivered by in vivo electroporation to B16 mouse
melanomas using two very different pulsing protocols. Reporter expression increased 21- or 42-fold, respectively with electroporation over injection alone. The growth of
experimental melanomas with an approximate diameter of 4 mm on the day of treatment was monitored after electroporation delivery of reporter plasmid
DNA. Remarkably, short-term complete regressions using one of these pulsing protocols occurred in up to 100% of mice. These regressions continued long term in up to 83% of animals. 70% of these mice were resistant to challenge with
B16 melanoma cells. Histological analysis revealed large numbers of apoptotic cells 24 h
after treatment. This antitumor effect did not require therapeutic
cDNA expression or eukaryotic sequences.