Abstract |
Excretion of 1,N(6)-ethenodeoxyadenosine (epsilon dA), a marker for lipid peroxidation (LPO)-derived DNA damage was analyzed in urine of nonsmoking postmenopausal women participating in a dietary intervention trial in Northern Japan. Hereby the efficacy of dietary consultation in reducing salt and increasing vitamin C and carotenes during 1 year was estimated. Thirty postmenopausal women, 60-69 years of age, from the intervention group and 30 age-matched women from the control group were randomly selected. The subjects completed a self-administered diet history questionnaire and in the pre- and post-intervention period 48 hr urine and fasting blood samples were collected. epsilon dA in urine was analyzed by an immuno-precipitation-high performance liquid chromatography-fluorescence detection method. epsilon dA excretion (/48 hr) in the 59 postmenopausal Japanese women with complete urine collection ranged from 12-226 pmol at the pre-intervention. At the pre-intervention, epsilon dA excretion was positively associated with urinary salt excretion (R = 0.33, p = 0.01) and omega-6 polyunsaturated fatty acid intake (%energy value, R = 0.28, p = 0.03) in the 59 women. The average epsilon dA excretion in the intervention group was 61 pmol at pre-intervention and 44 pmol at post-intervention (p = 0.14). In the control group, it was 58 pmol at pre-intervention and 75 pmol at post-intervention (p = 0.24). During the intervention period, 18/29 (62%) of the subjects in the intervention group exhibited the decreased excretion and 10/26 (38%) in the control group (p = 0.08). Results from this pilot study suggest urinary epsilon dA as a potential biomarker of DNA damage possibly derived from salt-induced inflammation and LPO; further exploration of epsilon dA in human biomonitoring studies is warranted.
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Authors | Tomoyuki Hanaoka, Jagadeesan Nair, Yoshiko Takahashi, Satoshi Sasaki, Helmut Bartsch, Shoichiro Tsugane |
Journal | International journal of cancer
(Int J Cancer)
Vol. 100
Issue 1
Pg. 71-5
(Jul 01 2002)
ISSN: 0020-7136 [Print] United States |
PMID | 12115589
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2002 Wiley-Liss, Inc. |
Chemical References |
- Biomarkers
- Deoxyadenosines
- Dietary Fats
- Fatty Acids, Omega-6
- Fatty Acids, Unsaturated
- Vitamins
- Sodium Chloride
- 1,N(6)-ethenodeoxyadenosine
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Topics |
- Aged
- Biomarkers
(urine)
- Chromatography, High Pressure Liquid
- Cross-Over Studies
- DNA Damage
- Dairy Products
- Deoxyadenosines
(urine)
- Diet
- Dietary Fats
(administration & dosage)
- Fatty Acids, Omega-6
- Fatty Acids, Unsaturated
(administration & dosage)
- Female
- Humans
- Immunosorbent Techniques
- Japan
- Lipid Peroxidation
- Middle Aged
- Oxidative Stress
- Postmenopause
- Sodium Chloride
(urine)
- Vitamins
(blood)
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