Abstract | BACKGROUND: METHODS: Peripheral blood samples from a total of 37 NPC patients with well-documented distant metastasis (M1) were collected before treatment. Eighteen patients had more than one blood sampling. Five different Taq DNA polymerases were used to test the blood from 17 patients. Peripheral blood of 37 nonmetastatic (M0) NPC patients was tested by the same nested RT-PCR system using multiple Taq DNA polymerases to evaluate the impact of multienzyme assay in the prediction of subsequent distant metastasis. RESULTS: Among M1 NPC patients, the accumulative positive rates of CK-19 mRNA were 22.2%, 44.4%, 70.6%, 75.0%, and 80.0% when one, two, three, four, or five blood sampling were taken, respectively. The accumulative positive rates increased as the numbers of different enzymes increased-from 35.5% by one enzyme to 82.4% by five enzymes. Six of 37 M0 patients had distant metastasis develop after a median follow-up time of 20 months. The detection sensitivity for four- enzyme test (5 of 6 = 83.3%) is better than that of one- enzyme test (2 of 6 = 33.3%). CONCLUSIONS: Our data demonstrate that multiple blood sampling or using multiple enzymes for nested RT-PCR assay significantly enhances the sensitivity in the molecular diagnosis of NPC metastasis.
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Authors | Jin-Ching Lin, Kuang Y Chen, Wen-Yi Wang, Jian-Sheng Jan, Yau-Huei Wei |
Journal | Head & neck
(Head Neck)
Vol. 24
Issue 6
Pg. 591-6
(Jun 2002)
ISSN: 1043-3074 [Print] United States |
PMID | 12112557
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2002 Wiley Periodicals, Inc. |
Chemical References |
- Biomarkers, Tumor
- RNA, Messenger
- RNA, Neoplasm
- Keratins
- DyNAzyme polymerase
- Taq Polymerase
- DNA-Directed DNA Polymerase
|
Topics |
- Adolescent
- Adult
- Aged
- Biomarkers, Tumor
(analysis)
- DNA-Directed DNA Polymerase
- Female
- Humans
- Keratins
(analysis, genetics)
- Male
- Middle Aged
- Nasopharyngeal Neoplasms
(blood, pathology)
- Neoplastic Cells, Circulating
- RNA, Messenger
(analysis)
- RNA, Neoplasm
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity
- Taq Polymerase
|