Molecular cloning and sequencing of feline melanoma antigen 2 (fMAGE-2) obtained from a lymphoma cell line.

Abstract

Melanoma antigens (MAGE), thought to induce tumor-specific immune responses, are used as potential therapeutical targets for cancer immunotherapy. We hereby report the cloning and sequencing of MAGE cDNA clone, called feline MAGE-2 (fMAGE-2), obtained from a lymphoma cell line. fMAGE-2 cDNA is 1535 base pairs (bp) in length and contains an open reading frame (ORF) of 1131 bp encoding a protein of 376 amino acids. The predicted amino acid sequence shows 45%, 32-42%, 44-47%, and 33% homology with feline MAGE-1, human MAGE-A, human MAGE-B, and human MAGE-C proteins, respectively. mRNA transcripts of fMAGE-2 were detected by RT-PCR in some feline tumors, as well as in testis of adult cat, but not in other normal tissues, indicating that the expression pattern of fMAGE-2 is similar to that of the human MAGE family genes in tumors and normal tissues.

• Authors: Z Ma, T S Khatlani, K Sasaki, M Okuda, H Inokuma, T Onishi
• Journal: Tissue antigens (Tissue Antigens) Vol. 59 Issue 2 Pg. 127-32 (Feb 2002) ISSN: 0001-2815 [Print] England
• PMID: 12028540 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens, Neoplasm
• Melanoma-Specific Antigens
• Neoplasm Proteins

Topics

• Amino Acid Sequence
• Animals
• Antigens, Neoplasm
• Base Sequence
• Cats
• Cloning, Molecular
• Lymphoma (immunology)
• Melanoma-Specific Antigens
• Molecular Sequence Data
• Neoplasm Proteins (analysis, genetics)
• Phylogeny
• Sequence Analysis, DNA
• Tumor Cells, Cultured