Abstract |
The adhesion molecule CEACAM1 (CD66a, BGP, C-CAM) is not only involved in maintaining normal tissue architecture, but also acts as a tumor suppressor in several experimental systems where loss of CEACAM1 expression results in enhanced tumor-cell growth and tumorigenicity. In order to further analyze the role of CEACAM1 in the development of breast cancer, we performed Western-blot analysis and immunohistochemistry with highly specific monoclonal antibodies in a cohort of 68 mammary carcinomas which had also been analyzed for expression of cell-cycle regulatory proteins cyclin D1, cyclin E, p16, p21, p27, Rb, and Rb2, as well as for steroid hormone receptor status, Ki67, and HER2/neu immunoreactivity. High CEACAM1 protein expression as found using both methods correlated significantly with expression of the retinoblastoma proteins Rb (P=0.004 and 0.013) and Rb2/p130 (P=0.003 and 0.007). In addition, we found a weak association of CEACAM1 expression with p27 protein levels (P=0.087 and 0.039), but with none of the other analyzed parameters. These results indicate the possibility of a functional link between cell-adhesion molecules and cell-cycle regulation that might play an important role in the development of mammary carcinomas.
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Authors | Ana-Maria Bamberger, Holger Kappes, Carola Methner, Gabriele Rieck, Jens Brümmer, Christoph Wagener, Thomas Löning, Karin Milde-Langosch |
Journal | Virchows Archiv : an international journal of pathology
(Virchows Arch)
Vol. 440
Issue 2
Pg. 139-144
(Feb 2002)
ISSN: 0945-6317 [Print] Germany |
PMID | 11964043
(Publication Type: Journal Article)
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Chemical References |
- Antigens, CD
- Antigens, Differentiation
- CD66 antigens
- Carcinoembryonic Antigen
- Carrier Proteins
- Ceacam1 protein, mouse
- Cell Adhesion Molecules
- Intracellular Signaling Peptides and Proteins
- Microfilament Proteins
- Muscle Proteins
- Retinoblastoma Protein
- Tagln protein, mouse
- Tumor Suppressor Proteins
- KDM5A protein, human
- Retinoblastoma-Binding Protein 2
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Topics |
- Antigens, CD
(biosynthesis)
- Antigens, Differentiation
(biosynthesis)
- Blotting, Western
- Breast Neoplasms
(metabolism, pathology)
- Carcinoembryonic Antigen
- Carcinoma
(metabolism, secondary)
- Carrier Proteins
(biosynthesis, genetics)
- Cell Adhesion Molecules
- Female
- Genes, Retinoblastoma
- Humans
- Immunohistochemistry
- Intracellular Signaling Peptides and Proteins
- Male
- Microfilament Proteins
(biosynthesis, genetics)
- Middle Aged
- Muscle Proteins
- Retinoblastoma Protein
(biosynthesis, genetics)
- Retinoblastoma-Binding Protein 2
- Tumor Suppressor Proteins
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