A novel peptide derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats.

Abstract

A tetrapeptide derivative PEP1261 [Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu], corresponding to residues 39-42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 microg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and beta-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This peptide derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of beta-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.

• Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Kalidindi V S Narayana Raju, Chandrasekar Gayathri, Natarajan Arutselvan, Rengarajulu Puvanakrishnan
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 229 Issue 1-2 Pg. 9-17 (Jan 2002) ISSN: 0300-8177 [Print] Netherlands
• PMID: 11936851 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Antioxidants
• Boc-Lys(Boc)-Arg-Asp-Ser(tBu)-OtBu
• Peptide Fragments
• Reactive Oxygen Species
• KRDS peptide
• Diclofenac
• Glucuronidase
• Lactoferrin

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (metabolism, therapeutic use)
• Antioxidants (metabolism, therapeutic use)
• Arthritis, Experimental (drug therapy)
• Bone and Bones (metabolism)
• Diclofenac (metabolism, pharmacology)
• Glucuronidase (metabolism)
• Lactoferrin (therapeutic use)
• Lysosomes (metabolism)
• Male
• Peptide Fragments (therapeutic use)
• Rats
• Rats, Wistar
• Reactive Oxygen Species (metabolism)