Abstract | BACKGROUND AND AIM: Accumulation and infiltration by neutrophil granulocytes is a prominent feature in the local inflammatory process in ulcerative colitis (UC). The present study was performed to evaluate human neutrophil lipocalin (HNL) as a specific neutrophil marker in the inflamed lesions of the colon and rectum in patients with colitis and proctitis. METHODS: The activity of intestinal neutrophils with respect to release of granule proteins was studied in 18 patients with UC (10 with colitis and eight with isolated proctitis) and in 18 healthy controls using perfusion fluid and biopsies from the sigmoid colon and rectum. The released amounts of the neutrophil granule proteins HNL and myeloperoxidase (MPO) were determined by radioimmunoassays, and the location of HNL and MPO in biopsies from colonic mucosa was examined by immunohistochemistry. RESULTS: CONCLUSION: We conclude that the increased release of HNL and MPO in colorectal perfusion fluids indicates neutrophil involvement in the local inflammatory process, and suggest that HNL may serve as a specific marker of intestinal neutrophil activation in UC. GM-CSF, and to some extent IL-8, may play a role in neutrophil accumulation and priming in this disease.
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Authors | M Carlson, Y Raab, L Sevéus, S Xu, R Hällgren, P Venge |
Journal | Gut
(Gut)
Vol. 50
Issue 4
Pg. 501-6
(Apr 2002)
ISSN: 0017-5749 [Print] England |
PMID | 11889070
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acute-Phase Proteins
- Biomarkers
- Carrier Proteins
- Interleukin-8
- LCN2 protein, human
- Lipocalin-2
- Lipocalins
- Oncogene Proteins
- Proto-Oncogene Proteins
- Granulocyte-Macrophage Colony-Stimulating Factor
- Peroxidase
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Topics |
- Acute-Phase Proteins
- Adult
- Aged
- Biomarkers
- Carrier Proteins
(metabolism)
- Colitis, Ulcerative
(metabolism)
- Female
- Granulocyte-Macrophage Colony-Stimulating Factor
(metabolism)
- Humans
- Immunohistochemistry
- Interleukin-8
(metabolism)
- Intestinal Mucosa
- Lipocalin-2
- Lipocalins
- Male
- Middle Aged
- Neutrophils
(metabolism)
- Oncogene Proteins
- Peroxidase
(metabolism)
- Proctitis
(metabolism)
- Proto-Oncogene Proteins
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