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Insulin-like growth factor-I-induced migration of melanoma cells is mediated by interleukin-8 induction.

Abstract
Successive events of growth factor-induced autocrine and paracrine activation promote tumor growth and metastasis. Insulin-like growth factor-I (IGF-I) stimulates melanoma cells to grow, survive, and migrate. Interleukin-8 (IL-8) is produced by melanoma cells and has been correlated with melanoma metastasis, but the biological functions of this cytokine have not been elucidated. We show here that IGF-I-induced migration of melanoma cells could be inhibited by neutralizing antibody against IL-8. IGF-I overexpression induced IL-8 production in melanoma cells, especially in biologically early melanomas by accelerating its transcription rate via activation of mitogen-activated protein kinase pathway. IGF-I treatment phosphorylated c-Jun and stimulated the binding of AP-1 but not NF-kappaB to the IL-8 promoter. These data identify IL-8 as a new target of IGF-I in melanoma and suggest that some of the biological functions of IGF-I are mediated by IL-8.
AuthorsKapaettu Satyamoorthy, Gang Li, Bhavesh Vaidya, Jiri Kalabis, Meenhard Herlyn
JournalCell growth & differentiation : the molecular biology journal of the American Association for Cancer Research (Cell Growth Differ) Vol. 13 Issue 2 Pg. 87-93 (Feb 2002) ISSN: 1044-9523 [Print] United States
PMID11864912 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antibodies, Monoclonal
  • DNA Primers
  • Insulin
  • Interleukin-8
  • RNA, Messenger
  • Insulin-Like Growth Factor I
Topics
  • Antibodies, Monoclonal
  • Blotting, Northern
  • Blotting, Western
  • Cell Movement (drug effects)
  • Cell Nucleus (metabolism)
  • DNA Primers (chemistry)
  • Electrophoretic Mobility Shift Assay
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • In Vitro Techniques
  • Insulin (metabolism)
  • Insulin-Like Growth Factor I (pharmacology)
  • Interleukin-8 (biosynthesis)
  • Melanoma (metabolism)
  • RNA, Messenger (metabolism)
  • Tumor Cells, Cultured (drug effects, metabolism)

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