Three-dimensional image of hepatocellular carcinoma under confocal laser scanning microscope.

Abstract

AIM:To investigate the application of confocal laser scanning microscopy (CLSM) in tumor pathology and three-dimensional (3-D) reconstruction by CLSM in pathologic specimens of hepatocellular carcinoma (HCC).METHODS:The 30&mgr;m thick sections were cut from the paraffin embedded tissues of HCC, hyperplasia and normal liver,stained with DNA fluorescent probe YOYO-1 iodide and examined by CLSM to collect optical sections of nuclei and 3-D images reconstructed.RESULTS:HCC displayed chaotic arrangement of carcinoma cell nuclei, marked pleomorphism, indented and irregular nuclear surface, and irregular and coarse chromatin texture.CONCLUSION:The serial optical tomograms of CLSM can be used to create 3-D reconstruction of cancer cell nuclei. Such 3-D impressions might be helpful or even essential in making an accurate diagnosis.

• Authors: Wang-Hai Zhang, Shi-Neng Zhu, Shi-Lun Lu, Ya-Lin Huang, Peng Zhao
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 6 Issue 3 Pg. 344-347 (Jun 2000) ISSN: 2219-2840 [Electronic] United States
• PMID: 11819594 (Publication Type: Journal Article)