Abstract | OBJECTIVE: METHODS: SiHa cells with overexpression of Bcl-2 (SiHa-Bcl2 cells) were established by transfecting SiHa cells with Bcl-2 expression vector. The sensitivities of SiHa and SiHa-Bcl2 cells to As2O3 were determined using MTT ( Thiazolyl blue) reduction and colony forming ability assay, morphological analysis, flow cytometric analysis, DNA agarose gel electrophoresis, in situ cell death detection (TUNEL), Northern blot, RT-PCR and Western blot. RESULTS:
As2O3 inhibited the growth of SiHa cells and induced G2/M arrest and apoptosis of the cells. RT-PCR and Western blot analysis revealed that As2O3 induced SiHa cell apoptosis possibly via inhibiting the expression of HPV16 E7 and decreasing the expression of c-myc. However, we found that SiHa-Bcl2 cells partly resisted As2O3 induced apoptosis, which might be related to the prevention of the down-regulation of HPV16 E7 and c-myc gene expression. Nevertheless, As2O3 at a high concentration could still induce apoptosis of SiHa-Bcl2 cells mainly via decreasing Bcl-2 expression and slightly inhibiting viral gene expression. CONCLUSION:
As2O3 is an inducer of the apoptosis of human cervical carcinoma cells and the cells overexpressing Bcl-2 can partly resist As2O3 induced apoptosis, but the exact mechanism is unclear.
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Authors | Y Deng, C Lin, J Zheng, M Fu, X Liang, J Chen, P Xiao, M Wu |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 113
Issue 1
Pg. 84-8
(Jan 2000)
ISSN: 0366-6999 [Print] China |
PMID | 11775218
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Arsenicals
- DNA, Neoplasm
- Oxides
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-bcl-2
- Tumor Suppressor Protein p53
- bcl-2-Associated X Protein
- Arsenic Trioxide
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Arsenic Trioxide
- Arsenicals
(pharmacology)
- Cell Cycle
(drug effects)
- Cell Survival
(drug effects)
- DNA, Neoplasm
(analysis)
- Female
- Humans
- Oxides
(pharmacology)
- Proto-Oncogene Proteins
(analysis)
- Proto-Oncogene Proteins c-bcl-2
(physiology)
- Tumor Suppressor Protein p53
(analysis)
- Uterine Cervical Neoplasms
(pathology)
- bcl-2-Associated X Protein
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