Time course profile and cell-type-specific production of monokine induced by interferon-gamma in Concanavalin A-induced hepatic injury in mice: comparative study with interferon-inducible protein-10.

Abstract

BACKGROUND:

We have previously shown that interferon-inducible protein-10 (IP-10), a chemokine for activated lymphocytes, was specifically induced in the liver of Concanavalin A (Con A)-treated mice. The aim of this study was to investigate the time course profile and cell-type-specific hepatic production of monokine induced by interferon-gamma (MIG), a chemokine which shares its receptor and most of its activity with IP-10, in Con A-treated mice and to compare them with those of IP-10.

METHODS:

Hepatic mRNA expression of MIG and IP-10 was studied by means of Northern blot analysis and in situ hybridization in Con A-treated mice. The levels of MIG and IP-10 in the serum and culture supernatants of murine hepatoma-, hepatic sinusoidal endothelial cell-, hepatic stellate cell- and macrophage-derived cell lines were determined by means of specific enzyme-linked immunosorbent assays.

RESULTS:

The serum level of MIG slowly reached a maximum at 12 h after Con A injection and remained elevated for a long time, whereas that of IP-10 reached a maximum at 3 h and declined quickly, a finding supported by Northern blot analysis. Using in situ hybridization, the mRNA of MIG as well as IP-10 was found to be expressed in hepatocytes and hepatic non-parenchymal cells. Similar to IP-10, MIG was produced by hepatoma-, hepatic sinusoidal endothelial cell-, hepatic stellate cell- and macrophage-derived cell lines in vitro.

CONCLUSIONS:

Although both MIG and IP-10 were produced by hepatocytes and hepatic non-parenchymal cells in Con A-treated mice, the time course profile of MIG was distinguishable from that of IP-10. The fact that hepatic MIG and IP-10 were produced sequentially in this hepatitis model may suggest that a non-redundant role is played by these two chemokines in the process of hepatic necro-inflammation.

• Authors: Y Itoh, A Morita, K Nishioji, H Fujii, H Nakamura, T Kirishima, T Toyama, N Yamauchi, Y Nagao, S Narumi, T Okanoue
• Journal: Scandinavian journal of gastroenterology (Scand J Gastroenterol) Vol. 36 Issue 12 Pg. 1344-51 (Dec 2001) ISSN: 0036-5521 [Print] England
• PMID: 11761028 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCL9 protein, human
• Chemokine CXCL10
• Chemokine CXCL9
• Chemokines, CXC
• Intercellular Signaling Peptides and Proteins
• Concanavalin A
• Interferon-gamma

Topics

• Animals
• Blotting, Northern
• Cell Line
• Chemokine CXCL10
• Chemokine CXCL9
• Chemokines, CXC (metabolism)
• Concanavalin A
• Enzyme-Linked Immunosorbent Assay
• Hepatitis, Animal (metabolism)
• In Situ Hybridization
• Intercellular Signaling Peptides and Proteins
• Interferon-gamma
• Liver (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Time Factors