Interleukin-1beta mediates ischemic injury in the rat retina.

Abstract

Two types of experiment were performed to examine the role of interleukin-1beta in ischemia-induced damage in the rat retina. In the in vivo study, enzyme-linked immunosorbent assay was used to investigate the expression of immunoreactive interleukin-1beta in the rat retina following a hypertension-induced ischemia/reperfusion, while the effect of a recombinant human interleukin-1 receptor antagonist or an anti-interleukin-1beta neutralizing antibody on the ischemia-induced damage was examined histologically. A transient increase in the expression of immunoreactive interleukin-1beta was observed in the retina 3-12 hr after reperfusion, and morphometric evaluation at 7 days after the ischemia showed a decrease in cell numbers in the ganglion cell layer and a decreased thickness of the inner plexiform layer with no change in the other retinal layers. Intravitreal injection of interleukin-1 receptor antagonist (1 or 10 ng per eye) or anti-interleukin-1beta antibody (50 or 500 ng per eye) 5 min before the onset of the ischemia reduced the damage. In the in vitro study, interleukin-1 receptor antagonist (500 ng ml(-1)) significantly reduced glutamate-induced neurotoxicity in rat cultured retinal neurons. These results suggest that interleukin-1 plays an important role in mediating ischemic and excitotoxic damage in the retina, and that interleukin-1 inhibitors may be therapeutically useful against neuronal injury caused by optic nerve or retinal diseases such as glaucoma and central retinal artery or vein occlusion.

• Authors: S Yoneda, H Tanihara, N Kido, Y Honda, W Goto, H Hara, N Miyawaki
• Journal: Experimental eye research (Exp Eye Res) Vol. 73 Issue 5 Pg. 661-7 (Nov 2001) ISSN: 0014-4835 [Print] England
• PMID: 11747366 (Publication Type: Comparative Study, Journal Article)
• Copyright: (C) 2001 Academic Press.

Chemical References

• Interleukin-1
• Glutamic Acid
• Dizocilpine Maleate

Topics

• Analysis of Variance
• Animals
• Cell Count
• Cell Survival (drug effects)
• Cells, Cultured (drug effects)
• Choroid Plexus (pathology)
• Dizocilpine Maleate (pharmacology)
• Enzyme-Linked Immunosorbent Assay
• Ganglia, Sensory (pathology)
• Glutamic Acid (pharmacology)
• Interleukin-1 (physiology)
• Male
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology)
• Retina (pathology)
• Retinal Diseases (metabolism, pathology)