Fluorescent imaging in a glioma model in vivo.

Abstract

BACKGROUND AND OBJECTIVE:

Nile blue dyes have been shown to have affinity for tumor tissue as compared to surrounding normal tissue and to be relatively non-toxic. We have employed EtNBA, a lipophilic, fluorescent benzophenoxazine dye, in a murine model to image subcutaneous and intracranial U-87 glioma implants.

STUDY DESIGN/MATERIALS AND METHODS:

The imaging system used to detect fluorescence consists of a SIT video camera fitted with a zoom microscope-magnifying lens. The tumor was illuminated with a 632.8-nm diffuse beam from a helium-neon laser. The video image was processed using a Sony image processor to give real-time pseudocolor and enhanced black and white images.

RESULTS:

Following subcutaneous injection of the dye at doses of 2.5-5.0 mg/kg bw, we observed a gradual increase of the fluorescent signal from the tumor which peaked 1-3 hours post-injection with variable selectivity (typically 4:1) for tumor to normal surrounding tissues permitting the clear demarcation of the tumor.

CONCLUSIONS:

The present in vivo study demonstrates that EtNBA is a safe and effective photodiagnostic agent, able to demarcate U87-MG solid tumors in mice on a real-time basis at a concentration of 2.5-5.0 mg/kg 1-3 hours after administration.

• Authors: D C Nikas, J W Foley, P M Black
• Journal: Lasers in surgery and medicine (Lasers Surg Med) Vol. 29 Issue 1 Pg. 11-7 ( 2001) ISSN: 0196-8092 [Print] United States
• PMID: 11500856 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2001 Wiley-Liss, Inc.

Chemical References

• Fluorescent Dyes
• Oxazines
• 5-ethylamino-9- diethylaminobenzo(a)phenoxazinium

Topics

• Animals
• Brain Neoplasms (diagnosis, pathology)
• Fluorescence
• Fluorescent Dyes (administration & dosage)
• Glioma (diagnosis, pathology)
• Injections, Subcutaneous
• Male
• Mice
• Mice, Nude
• Microscopy, Fluorescence
• Neoplasm Transplantation
• Oxazines (administration & dosage)
• Time Factors