Abstract | BACKGROUND/AIMS: METHODS: The first model included 18 spontaneously hypertensive rats studied at 4, 10, and 16 weeks of age. The second model included 28 Wistar rats with two-kidney, one- clip Goldblatt hypertension studied at 7, 14, 21, and 28 days. The third model included 6 Wistar rats treated with N(G)-nitro-L-arginine methyl ester during 10 days. Respective controls were studied for all models. At the end of each experimental period, the systolic blood pressure was measured in the tail by plethysmography. Individual renal cortex total RNA was extracted, and the mRNA levels of the thiazide-sensitive Na+:Cl- cotransporter were assessed following a semiquantitative RT-PCR strategy. RESULTS: All experimental models developed systemic hypertension. However, the level of mRNA expression of the Na+:Cl- cotransporter did not change in any of the models studied as compared with their respective controls. CONCLUSION:
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Authors | G Moreno, N A Bobadilla, J González-Salazar, A Mercado, E Tapia, E Hong, J Herrera-Acosta, G Gamba |
Journal | Kidney & blood pressure research
(Kidney Blood Press Res)
Vol. 24
Issue 1
Pg. 57-63
( 2001)
ISSN: 1420-4096 [Print] Switzerland |
PMID | 11174008
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzothiadiazines
- Carrier Proteins
- Diuretics
- RNA, Messenger
- Receptors, Drug
- Slc12a3 protein, rat
- Sodium Chloride Symporter Inhibitors
- Sodium Chloride Symporters
- Solute Carrier Family 12, Member 3
- Symporters
- thiazide receptor
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Topics |
- Animals
- Benzothiadiazines
- Carrier Proteins
(genetics, metabolism)
- Disease Models, Animal
- Diuretics
- Gene Expression Regulation
- Hypertension
(genetics, metabolism, physiopathology)
- Hypertension, Renovascular
(genetics, metabolism, physiopathology)
- RNA, Messenger
(biosynthesis, genetics)
- Rats
- Rats, Inbred SHR
- Rats, Inbred WKY
- Rats, Wistar
- Receptors, Drug
(genetics, metabolism)
- Sodium Chloride Symporter Inhibitors
(metabolism)
- Sodium Chloride Symporters
- Solute Carrier Family 12, Member 3
- Symporters
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