Identification of a novel autoantigen UACA in patients with panuveitis.

Abstract	To identify the target autoantigens in Vogt-Koyanagi-Harada disease, we made use of an immunoscreening of a bovine uveal cDNA expression library with serum samples obtained from patients with Vogt-Koyanagi-Harada disease. We identified a novel bovine antigen and homologous human autoantigen and designated it as UACA (uveal autoantigen with coiled coil domains and ankyrin repeats). mRNA of human UACA is expressed most abundantly in skeletal muscles and in various human tissues, including choroid, retina, and epidermal melanocytes. IgG autoantibodies were quantitated in an ELISA, using recombinant C-terminal 18.0% fragment of human UACA. The prevalence of IgG anti-UACA autoantibodies in patients with panuveitis (Vogt-Koyanagi-Harada disease, Behçet's disease, sarcoidosis) was significantly higher than that in healthy controls (19.6-28.1% vs 0%, P < 0.05) indicating that autoimmunity directed against UACA is a common phenomenon in these diseases.
Authors	K Yamada, S Senju, T Nakatsura, Y Murata, M Ishihara, S Nakamura, S Ohno, A Negi, Y Nishimura
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 280 Issue 4 Pg. 1169-76 (Feb 02 2001) ISSN: 0006-291X [Print] United States
PMID	11162650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright 2001 Academic Press.
Chemical References	Autoantigens DNA, Complementary Immunoglobulin G RNA, Messenger Recombinant Fusion Proteins Recombinant Proteins UACA antigen UACA antigen, bovine Glutathione Transferase
Topics	Adolescent Adult Aged Aged, 80 and over Amino Acid Sequence Animals Autoantigens (biosynthesis, chemistry, immunology) Blotting, Northern Cattle Choroid (metabolism) DNA, Complementary (metabolism) Enzyme-Linked Immunosorbent Assay Female Gene Library Glutathione Transferase (metabolism) Humans Immunoglobulin G (metabolism) Male Middle Aged Molecular Sequence Data Panuveitis (immunology) RNA, Messenger (metabolism) Recombinant Fusion Proteins (metabolism) Recombinant Proteins (metabolism) Retina (metabolism) Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Tissue Distribution

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