Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey.

Abstract

OBJECTIVES:

The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is an important protein for immortalization and tumorigenesis of infected cells. EBNA-1 gene variants may play a role in tumorigenesis. We determined the nucleotide and amino acid (aa) sequences of EBNA-1 in EBV-related herpesviruses from cynomolgus monkeys (cynomolgus-EBV) which induced malignant lymphomas in its natural host and in rabbits, and compared them with sequences of EBV and other lymphocryptoviruses (LCVs).

METHODS:

Polymerase chain reaction and direct sequencing methods were performed using extracted DNA from cynomolgus-EBV-infected cell lines.

RESULTS:

The amino acid sequences of cynomolgus-EBV EBNA-1 from two cell lines (Si-IIA: 588 aa; Ts-B6: 619 aa) which are antigenically cross-reactive to human EBV EBNA-1 showed homology with human EBV (Si-IIA: 53%; Ts-B6: 58%) and other LCVs from baboons (54 and 52%) and rhesus monkeys (60 and 58%), especially in the C-terminal unique domain. Homology of the EBNA-1 sequence between Si-IIA and Ts-B6 was 92%. The sequence difference between EBV and the related LCVs was manifested mainly in the length of the internal repeat 3-corresponding region, which contains serine in the glycine/alanine repeat region of nonhuman LCVs.

CONCLUSION:

Sequence variation of cynomolgus-EBV EBNA-1 from different cell lines was observed. However, their sequences show a relatively high homology with human EBV and share the common features of EBNA-1 of EBV and other LCVs.

• Authors: N Ohara, K Hayashi, N Teramoto, T Oka, K Fujimoto, Y Yoshikawa, E Castanos-Velez, P Biberfeld, T Akagi
• Journal: Intervirology (Intervirology) Vol. 43 Issue 2 Pg. 102-6 ( 2000) ISSN: 0300-5526 [Print] Switzerland
• PMID: 10971128 (Publication Type: Journal Article)
• Copyright: Copyright 2000 S. Karger AG, Basel

Chemical References

• Epstein-Barr Virus Nuclear Antigens

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Cell Line, Transformed
• Epstein-Barr Virus Nuclear Antigens (chemistry, genetics)
• Genetic Variation
• Herpesvirus 4, Human (genetics, immunology)
• Humans
• Lymphocryptovirus (genetics, immunology)
• Macaca fascicularis (virology)
• Molecular Sequence Data
• Phylogeny
• Rabbits
• Sequence Analysis, DNA