Abstract | BACKGROUND: We hypothesize that dendritic cells (DCs) can process antigens from autologous melanoma apoptotic bodies (MABs) and induce effector T cells in melanoma patients. MATERIALS AND METHODS: Peripheral blood mononuclear cells were obtained from three stage IV melanoma patients and adherent cells were cultured in complete medium (CM) containing GM-CSF (800 U/ml) and IL-4 (1000 U/ml) for 7 days. Autologous MABs from melanoma cells following actinomycin D treatment (0.5 microgram/ml) for 24 hours, were added to 72 hour DC culture. Autologous effector T cells were cultured in CM containing 60 IU/ml of IL-2 and were stimulated by MAB-pulsed DCs three times at a weekly interval. Effector T cells were harvested at the end of third cycle of DC stimulation. RESULTS: Using ELISPOT, IFN-gamma production by effector T cells stimulated by MAB-pulsed DCs was significantly higher than that by T cells without DC stimulation. Microscopy demonstrated phagocytosis of MABs by DCs. CONCLUSIONS: MAB-pulsed DCs are capable of stimulating Th1-directed autologous effector T cells. Pulsing DCs with autologous MABs may be a novel approach in future DC-based immunotherapeutic trials.
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Authors | J W Chang, M Peng, J E Vaquerano, Y M Zhou, R A Clinton, W C Hyun, M A Giedlin, S P Leong |
Journal | Anticancer research
(Anticancer Res)
2000 May-Jun
Vol. 20
Issue 3A
Pg. 1329-36
ISSN: 0250-7005 [Print] Greece |
PMID | 10928040
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Neoplasm
- Interleukin-10
- Interferon-gamma
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Topics |
- Antigens, Neoplasm
(immunology)
- Apoptosis
(immunology)
- Dendritic Cells
(immunology, physiology)
- Humans
- In Vitro Techniques
- Interferon-gamma
(analysis)
- Interleukin-10
(analysis)
- Lymphocyte Activation
(immunology)
- Melanoma
(pathology)
- Phagocytosis
- Phenotype
- T-Lymphocytes
(immunology)
- Th1 Cells
(immunology)
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