Abstract | BACKGROUND: Cell death by apoptosis seems to be an important mechanism for translocation to the cell surface of a variety of intracellular components capable of inducing autoantibody production. AIMS: METHODS: RESULTS: Three patterns of scanning laser immunofluorescence microscopy in non-apoptotic neutrophils were observed with respect to cellular ulcerative colitis associated ANCA distribution: (1) diffuse nuclear localisation (16.7%); (2) nuclear localisation in the nuclear periphery (50%); and (3) mixed nuclear and cytoplasmic localisation (33.4%). In all sera ANCA fluorescence colocalised almost completely with apoptotic DNA, with persistence of a diffuse and intense fluorescence. No significant changes in ANCA titres were found in non-apoptotic neutrophils. CONCLUSIONS: The antigen(s) of ANCA associated with ulcerative colitis seems to be localised in most cases in the neutrophil nucleus. The almost identical colocalisation of ANCA and apoptotic cleaved DNA suggests that intracellular DNA redistribution during neutrophil apoptosis may play a role in antigen exposure to the immune system and ANCA production in ulcerative colitis.
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Authors | J Mallolas, M Esteve, E Rius, E Cabré, M A Gassull |
Journal | Gut
(Gut)
Vol. 47
Issue 1
Pg. 74-8
(Jul 2000)
ISSN: 0017-5749 [Print] England |
PMID | 10861267
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Antineutrophil Cytoplasmic
- Autoantigens
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Topics |
- Antibodies, Antineutrophil Cytoplasmic
(immunology)
- Antigen-Antibody Reactions
(immunology)
- Apoptosis
(immunology)
- Autoantigens
(immunology)
- Cell Culture Techniques
- Cell Nucleus
(immunology)
- Colitis, Ulcerative
(immunology)
- Fluorescent Antibody Technique, Indirect
- Humans
- Microscopy, Confocal
- Microscopy, Fluorescence
- Neutrophils
(immunology)
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