Abstract | BACKGROUND: METHODS: Sixty-five male Wistar rats were divided into 5 groups of 13 animals each. Specimens for bacteriologic examination were taken, and pancreatitis was induced by intraductal infusion of 3% taurocholate under sterile conditions. Animals were killed 8, 16, 24, or 32 hours thereafter, and bacteriologic examination was performed. A control group of animals with intraductal infusion of 0.9% saline solution were killed after 32 hours. RESULTS: There was no significant pancreatic infection in the control group and in the 8-hour group (1 of 13 rats). Sixteen and 24 hours after induction of pancreatitis, infection and inflammation of the pancreas were found in 77% (10 of 13 rats), and after 32 hours pancreatic infection occurred in 69% (9 of 13 rats). Extrapancreatic bacterial infection after 16 hours occurred in the liver (62%), spleen (62%), and mesenteric lymph nodes (46%). Bacteria infecting the pancreas reflected the bacterial spectrum of the large bowel and terminal ileum before induction of pancreatitis (Escherichia coli [77%], Proteus [43%], Enterococcus [37%], and Staphylococcus [23%]). CONCLUSIONS: Pancreatic infection is an early and frequent finding in the taurocholate model of acute necrotizing pancreatitis. Infection occurs between 8 and 16 hours after induction of pancreatitis. The source of infecting bacteria seems to be the large bowel or the terminal ileum. We present a useful model of severe pancreatitis in which to study bacterial translocation, the further route of spread, and therapeutic approaches.
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Authors | M Schwarz, J Thomsen, H Meyer, M W Büchler, H G Beger |
Journal | Surgery
(Surgery)
Vol. 127
Issue 4
Pg. 427-32
(Apr 2000)
ISSN: 0039-6060 [Print] United States |
PMID | 10776434
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Acute Disease
- Animals
- Bacterial Infections
(etiology, physiopathology)
- Enterococcus
(isolation & purification)
- Escherichia coli
(isolation & purification)
- Escherichia coli Infections
(etiology, physiopathology)
- Hemorrhage
- Leukocytes
(pathology)
- Male
- Necrosis
- Pancreas
(microbiology, pathology)
- Pancreatic Diseases
(etiology, microbiology)
- Pancreatitis
(chemically induced, complications, microbiology)
- Proteus mirabilis
(isolation & purification)
- Rats
- Rats, Wistar
- Staphylococcal Infections
(etiology, physiopathology)
- Staphylococcus
(isolation & purification)
- Streptococcus agalactiae
(isolation & purification)
- Taurocholic Acid
(toxicity)
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