VEGF deprivation-induced apoptosis is a component of programmed capillary regression.

Abstract

The pupillary membrane (PM) is a transient ocular capillary network, which can serve as a model system in which to study the mechanism of capillary regression. Previous work has shown that there is a tight correlation between the cessation of blood flow in a capillary segment and the appearance of apoptotic capillary cells throughout the segment. This pattern of cell death is referred to as synchronous apoptosis (Lang, R. A., Lustig, M., Francois, F., Sellinger, M. and Plesken, H. (1994) Development 120, 3395-3404; Meeson, A., Palmer, M., Calfon, M. and Lang, R. A. (1996) Development 122, 3929-3938). In the present study, we have investigated whether the cause of synchronous apoptosis might be a segmental deficiency of either oxygen or a survival factor. Labeling with the compound EF5 in a normal PM indicated no segmental hypoxia; this argued that oxygen deprivation was unlikely to be the cause of synchronous apoptosis. When rat plasma was used as a source of survival factors in an in vitro PM explant assay, inhibition of vascular endothelial growth factor (VEGF) all but eliminated the activity of plasma in suppressing apoptosis. This argued that VEGF was an important plasma survival factor. Furthermore, inhibition of VEGF in vivo using fusion proteins of the human Flk-1/KDR receptor resulted in a significantly increased number of capillaries showing synchronous apoptosis. This provides evidence that VEGF is necessary for endothelial cell survival in this system and in addition, that VEGF deprivation mediated by flow cessation is a component of synchronous apoptosis.

• Authors: A P Meeson, M Argilla, K Ko, L Witte, R A Lang
• Journal: Development (Cambridge, England) (Development) Vol. 126 Issue 7 Pg. 1407-15 (Apr 1999) ISSN: 0950-1991 [Print] England
• PMID: 10068634 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Endothelial Growth Factors
• Hydrocarbons, Fluorinated
• Lymphokines
• Receptors, Growth Factor
• Recombinant Fusion Proteins
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors
• Etanidazole
• 2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide
• Receptor Protein-Tyrosine Kinases
• Receptors, Vascular Endothelial Growth Factor

Topics

• Animals
• Apoptosis (drug effects)
• Capillaries (drug effects, physiology)
• Cell Hypoxia
• Endothelial Growth Factors (antagonists & inhibitors, deficiency, pharmacology)
• Etanidazole (analogs & derivatives, metabolism)
• Hydrocarbons, Fluorinated (metabolism)
• Immunohistochemistry
• In Situ Nick-End Labeling
• Iris (blood supply, drug effects)
• Lymphokines (antagonists & inhibitors, deficiency, pharmacology)
• Mice
• Organ Culture Techniques
• Rats
• Rats, Sprague-Dawley
• Receptor Protein-Tyrosine Kinases (metabolism)
• Receptors, Growth Factor (metabolism)
• Receptors, Vascular Endothelial Growth Factor
• Recombinant Fusion Proteins (genetics, pharmacology)
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors