Staurosporine enhanced benzamide riboside-induced apoptosis in human multidrug-resistant promyelocytic leukemia cells (HL-60/VCR) in vitro.

Abstract

The inosine monophosphate (IMP) dehydrogenase inhibitor benzamide riboside (BR) induced apoptosis (detected with the aid of flow cytometric identification of cells with sub-G0 DNA content and increased side angle light scatter) equally or slightly more intensively in the multidrug-resistant human promyelocytic leukemia cell line (HL-60/VCR: MDR-1 gene, Pgp positive) in comparison with the parental drug sensitive HL-60 cells. Staurosporine alone induced relatively low level of apoptosis in parental HL-60 cells but higher level (approximately 35%) of apoptosis in multidrug-resistant HL-60/VCR cells after 24 hour induction. The combination of benzamide riboside and staurosporine induced in both drug-sensitive and drug-resistant HL-60 cells a marked proportion of apoptotic cells already after short (6 hour) induction (more than 30% of apoptotic cells).

• Authors: L Hunáková, J Duraj, D Romanová, L Novotný, J Sedlák, M R Kelley, T Szekeres, H N Jayaram, B Chorváth
• Journal: Neoplasma (Neoplasma) Vol. 45 Issue 4 Pg. 204-9 ( 1998) ISSN: 0028-2685 [Print] Slovakia
• PMID: 9890662 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Enzyme Inhibitors
• Neoplasm Proteins
• Nucleosides
• 3-(1-deoxyribofuranosyl)benzamide
• IMP Dehydrogenase
• Staurosporine

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (metabolism)
• Apoptosis (drug effects)
• DNA Fragmentation
• Drug Resistance, Multiple
• Drug Resistance, Neoplasm
• Drug Synergism
• Enzyme Inhibitors (pharmacology)
• Flow Cytometry
• HL-60 Cells (drug effects)
• Humans
• IMP Dehydrogenase (antagonists & inhibitors)
• Leukemia, Promyelocytic, Acute (pathology)
• Neoplasm Proteins (metabolism)
• Nucleosides (pharmacology)
• Staurosporine (pharmacology)