Ectopic expression of cdc2/cdc28 kinase subunit Homo sapiens 1 uncouples cyclin B metabolism from the mitotic spindle cell cycle checkpoint.

Abstract	Primary human fibroblasts arrest growth in response to the inhibition of mitosis by mitotic spindle-depolymerizing drugs. We show that the mechanism of mitotic arrest is transient and implicates a decrease in the expression of cdc2/cdc28 kinase subunit Homo sapiens 1 (CKsHs1) and a delay in the metabolism of cyclin B. Primary human fibroblasts infected with a retroviral vector that drives the expression of a mutant p53 protein failed to downregulate CKsHs1 expression, degraded cyclin B despite the absence of chromosomal segregation, and underwent DNA endoreduplication. In addition, ectopic expression of CKsHs1 interfered with the control of cyclin B metabolism by the mitotic spindle cell cycle checkpoint and resulted in a higher tendency to undergo DNA endoreduplication. These results demonstrate that an altered regulation of CKsHs1 and cyclin B in cells that carry mutant p53 undermines the mitotic spindle cell cycle checkpoint and facilitates the development of aneuploidy. These data may contribute to the understanding of the origin of heteroploidy in mutant p53 cells.
Authors	M L Hixon, A I Flores, M W Wagner, A Gualberto
Journal	Molecular and cellular biology (Mol Cell Biol) Vol. 18 Issue 11 Pg. 6224-37 (Nov 1998) ISSN: 0270-7306 [Print] United States
PMID	9774639 (Publication Type: Journal Article)
Chemical References	CKS1B protein, human Carrier Proteins Cell Cycle Proteins Cyclin B RNA, Messenger Tumor Suppressor Protein p53 Protein Kinases CDC2 Protein Kinase CDC2-CDC28 Kinases CDC28 Protein Kinase, S cerevisiae Cyclin-Dependent Kinases
Topics	CDC2 Protein Kinase (physiology) CDC2-CDC28 Kinases CDC28 Protein Kinase, S cerevisiae (physiology) Carrier Proteins (genetics) Cell Cycle (physiology) Cell Cycle Proteins Cyclin B (metabolism) Cyclin-Dependent Kinases DNA Replication (genetics) Fibroblasts Flow Cytometry Gene Expression Regulation (genetics) Genes, Viral (genetics) Humans Mitosis (physiology) Papillomaviridae (genetics) Ploidies Protein Kinases RNA, Messenger (metabolism) Spindle Apparatus (physiology) Transfection (genetics) Tumor Suppressor Protein p53 (genetics)

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