Elevation of mouse liver glutathione level by low-dose gamma-ray irradiation and its effect on CCl4-induced liver damage.

We examined the elevation of glutathione (GSH) levels in the liver of C57BL/6 female mice after low-dose r-ray irradiation and its inhibitory effect on CClI4-induced liver damage. The liver GSH level increased soon after irradiation with 50 cGy of gamma-rays, reached a maximum at around 12 post-treatment, and returned almost to the control level by 24 h. The activities of glutathione reductase, and glutathione peroxidase also showed the same pattern of change, while the activity of gamma-glutamylcysteine synthetase showed a gradual increase up to 24 h. The effect of pre-irradiation on CCl4-induced liver damage was also investigated. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in serum were markedly increased 12 h post-treatment with CCl4. Both increases were significantly suppressed by a single low-dose pre-irradiation. Malondialdehyde, a marker of lipidperoxidation, was also greatly elevated after CCl4 treatment, and its increase was suppressed by irradiation. These results suggest low-dose gamma-ray irradiation might be effective for the prevention of and/or therapy of various reactive oxygen species-related diseases including cancer.
AuthorsS Kojima, O Matsuki, T Nomura, A Kubodera, K Yamaoka
JournalAnticancer research (Anticancer Res) 1998 Jul-Aug Vol. 18 Issue 4A Pg. 2471-6 ISSN: 0250-7005 [Print] GREECE
PMID9703894 (Publication Type: Journal Article)
Chemical References
  • Glutathione Peroxidase
  • Glutathione Reductase
  • Glutamate-Cysteine Ligase
  • Glutathione
  • Animals
  • Carbon Tetrachloride Poisoning (metabolism, pathology)
  • Female
  • Gamma Rays
  • Glutamate-Cysteine Ligase (metabolism, radiation effects)
  • Glutathione (metabolism, radiation effects)
  • Glutathione Peroxidase (metabolism, radiation effects)
  • Glutathione Reductase (metabolism, radiation effects)
  • Kinetics
  • Liver (metabolism, pathology, radiation effects)
  • Mice
  • Mice, Inbred C57BL
  • Time Factors

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