Abstract | PURPOSE: METHODS: Corneal endothelial cell cultures were prepared from New Zealand white rabbits, using standard microcarrier technique. Two free-radical-generating systems were used-17 IU/L xanthine oxidase/1 mM hypoxanthine and 5 mM 3-morpholinosydnonimine-N-ethylcarbamide (SIN-1, a nitric oxide-donating agent). RESULTS: Over 95% of cultured corneal endothelial cells necrosed within 3.6 +/- 1.5 min after exposure to xanthine oxidase/ hypoxanthine. Adding morin hydrate delayed cell necrosis to 5.8 +/- 0.3 min (0.25 mM morin hydrate), 13.3 +/- 5.0 min (0.5 mM), and 41.5 +/- 8.6 min (1.0 mM). Exposed to nitric oxide generated by SIN-1, cells necrosed by 9.5 +/- 2.5 min, versus 14.1 +/- 1.3 min (0.25 mM morin hydrate), 27.2 +/- 2.0 min (0.5 mM), and 43.3 +/- 5.4 min (1.0 mM). Morin hydrate significantly prolonged survival of cells compared to equimolar concentrations of purpurogallin, Trolox, or ascorbate (P < 0.01). CONCLUSION:
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Authors | L H Zeng, D S Rootman, A Burnstein, J Wu, T W Wu |
Journal | Current eye research
(Curr Eye Res)
Vol. 17
Issue 2
Pg. 149-52
(Feb 1998)
ISSN: 0271-3683 [Print] England |
PMID | 9523092
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Benzocycloheptenes
- Chromans
- Flavonoids
- Free Radical Scavengers
- Free Radicals
- Hypoxanthine
- linsidomine
- morin
- Molsidomine
- Xanthine Oxidase
- purpurogallin
- 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Benzocycloheptenes
(pharmacology)
- Cell Survival
(drug effects)
- Cells, Cultured
- Chromans
(pharmacology)
- Cytoprotection
(drug effects)
- Endothelium, Corneal
(cytology, drug effects)
- Flavonoids
(pharmacology)
- Free Radical Scavengers
(pharmacology)
- Free Radicals
(metabolism)
- Hypoxanthine
(toxicity)
- Molsidomine
(analogs & derivatives, toxicity)
- Rabbits
- Xanthine Oxidase
(toxicity)
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