Cell cultures in the investigation of thiazide phototoxicity.

Abstract

The NHIK 3025 cell line (Norsk Hydro Institutt for Kreftforskning), a human in situ carcinoma of the cervix cell line, was used to investigate the thiazides bemetizide, bendroflumethiazide, benzylhydrochlorothiazide, bumetanide, butizide, chlortalidone, furosemide, hydrochlorothiazide, hydroflumethiazide, indapamide, piretanide, polythiazide, trichlormethiazide and xipamide for their potential phototoxic properties. Cell death following UVA irradiation and dependent on test substance concentration was observed in the presence of all the tested substances except chlortalidone, furosemide, indapamide and xipamide. Bendroflumethiazide was phototoxic at concentrations of 0.05 mM and higher; bemetizide, benzylhydrochlorothiazide, bumetanide and hydroflumethiazide were phototoxic at 0.25 mM and higher and butizide, hydrochlorothiazide, piretanide, polythiazide and trichlormethiazide were phototoxic at 0.5 mM and higher.

• Authors: E Selvaag
• Journal: Naunyn-Schmiedeberg's archives of pharmacology (Naunyn Schmiedebergs Arch Pharmacol) Vol. 356 Issue 3 Pg. 293-6 (Sep 1997) ISSN: 0028-1298 [Print] Germany
• PMID: 9303564 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Benzothiadiazines
• Diuretics
• Photosensitizing Agents
• Sodium Chloride Symporter Inhibitors

Topics

• Benzothiadiazines
• Cell Death (drug effects, radiation effects)
• Diuretics
• Dose-Response Relationship, Drug
• Dose-Response Relationship, Radiation
• Humans
• Photosensitizing Agents (toxicity)
• Sodium Chloride Symporter Inhibitors (toxicity)
• Tumor Cells, Cultured
• Ultraviolet Rays