Persistent elevation of immunoglobulin G titer against the C region of recombinant group A streptococcal M protein in patients with rheumatic fever.

Abstract

To analyze the immune response to the C region of group A streptococcal M protein in patients with rheumatic fever (RF), we cloned the structural gene for the C region of type 12 M protein and produced recombinant C region of M protein. IgG titers against the C region of M protein were measured by ELISA from sera of patients with RF (n = 10), uncomplicated streptococcal pharyngitis (n = 26), and age-matched controls (n = 49). IgG titers against the C region were significantly higher in patients with RF than in controls or patients with uncomplicated streptococcal pharyngitis (43 versus 1.5 or 1.8 micrograms/mL, p < 0.01). Studies using overlapping synthetic peptides of the C region demonstrated that increased IgG reactivity was observed against the amino-terminal halves of the C repeat blocks (C1, C2) in RF, indicating that these domains are the main immunodominant epitopes in the C region.

• Authors: K Mori, N Kamikawaji, T Sasazuki
• Journal: Pediatric research (Pediatr Res) Vol. 39 Issue 2 Pg. 336-42 (Feb 1996) ISSN: 0031-3998 [Print] United States
• PMID: 8825809 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Outer Membrane Proteins
• Bacterial Proteins
• Carrier Proteins
• DNA, Bacterial
• Immunoglobulin G
• Recombinant Fusion Proteins
• streptococcal M protein

Topics

• Amino Acid Sequence
• Antibodies, Bacterial (immunology)
• Antigens, Bacterial
• Bacterial Outer Membrane Proteins
• Bacterial Proteins (genetics, immunology)
• Base Sequence
• Carrier Proteins
• Child
• DNA, Bacterial (genetics)
• Epitope Mapping
• Female
• Gene Expression Regulation, Bacterial
• Humans
• Immunoglobulin G (immunology)
• Male
• Molecular Sequence Data
• Recombinant Fusion Proteins (immunology)
• Rheumatic Fever (immunology)