Differential diagnoses of influenza A virus, influenza B virus, and respiratory syncytial virus infections by direct immunofluorescence using mixtures of monoclonal antibodies of different isotypes.

Abstract

Differences between isotypes of monoclonal antibodies were employed to detect influenza A and B viruses and respiratory syncytial virus by direct immunofluorescence using fluorescein isothiocyanate or Texas Red conjugates. Examination of 56 specimens for influenza A and B viruses and 112 specimens for influenza A virus and respiratory syncytial virus showed the mixed-isotype test to be comparable to the conventional procedure.

• Authors: P Murphy, Z M Roberts, J L Waner
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 34 Issue 7 Pg. 1798-800 (Jul 1996) ISSN: 0095-1137 [Print] United States
• PMID: 8784593 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Fluorescent Dyes
• Immunoglobulin Isotypes
• Xanthenes
• Texas red
• Fluorescein-5-isothiocyanate

Topics

• Antibodies, Monoclonal
• Antibodies, Viral
• Diagnosis, Differential
• Evaluation Studies as Topic
• Fluorescein-5-isothiocyanate
• Fluorescent Antibody Technique, Direct (methods)
• Fluorescent Dyes
• Humans
• Immunoglobulin Isotypes
• Influenza A virus (immunology)
• Influenza B virus (immunology)
• Influenza, Human (diagnosis, virology)
• Respiratory Syncytial Virus Infections (diagnosis)
• Respiratory Syncytial Viruses (immunology)
• Xanthenes