[Effects of a platelet-activating factor antagonist ONO-6240 on antigen induced eosinophil infiltration into the airways].

Abstract

An in vivo mouse model of allergic bronchial asthma was developed to investigate the effects of a platelet activating factor antagonist ONO-6240 on eosinophil (EOS) recruitment into the mouse airway. In the present study, no EOS could be found in bronchoalveolar lavage fluid (BALF) from normal control mice. However, chronic ovalbumin challenge of sensitized mice induced significant bronchoalveolar eosinophilia (9.68 +/- 0.72 x 10(8)/L). In three groups of mice treated intraperitoneally with different doses of ONO-6240 (0.1, 1.0, 10.0 mg/kg), the medication produced 42.1% (5.60 +/- 0.97 x 10(8)/L, P < 0.01), 58.8% (3.99 +/- 0.84 x 10(8)/L, P < 0.01), and 72.6% (2.65 +/- 0.71 x 10(8)/L, P < 0.01) inhibition, respectively, of the antigen-induced influx of EOS into BALF. It was found that the prevention of EOS infiltration into airway with ONO-6240 was accompanied by decrements of both interleukin (IL)-5 and IL-2 levels. These data indicated that ONO-6240 prevented airway eosinophilia by inhibiting production of IL-5 and IL-2. Our results suggested that ONO-6240 may be of value in treating human asthmatic patients.

• Authors: H Shi, H Xu, G Liang
• Journal: Zhonghua nei ke za zhi (Zhonghua Nei Ke Za Zhi) Vol. 34 Issue 11 Pg. 753-6 (Nov 1995) ISSN: 0578-1426 [Print] China
• PMID: 8731847 (Publication Type: English Abstract, Journal Article)

Chemical References

• Interleukin-2
• Interleukin-5
• Platelet Activating Factor
• Thiazoles
• ONO 6240

Topics

• Animals
• Asthma (pathology)
• Blood Cell Count (drug effects)
• Bronchoalveolar Lavage Fluid (chemistry, cytology)
• Eosinophils (cytology)
• Female
• Interleukin-2 (metabolism)
• Interleukin-5 (metabolism)
• Mice
• Mice, Inbred BALB C
• Platelet Activating Factor (antagonists & inhibitors)
• Thiazoles (pharmacology)