Column cation-exchange separation of melanin-related metabolites in urine from cases of melanoma.

Abstract

We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma urines. A procedure for buffering aqueous/solvent reagent solutions is presented, and examples of the baseline stability achieved for reference chromatograms and urine samples are provided. Chromatograms of phaeomelanin precursors and of an extract of a highly pigmented hamster melanoma are also presented. Identities are tentatively assigned for some of the chromatographic peaks that have previously been correlated with disease, including isomers of cysteinyldopa, and observations of new pigment- and tumor-related metabolites in the chromatograms are noted.

• Authors: P W Banda, A E Sherry, M S Blois
• Journal: Clinical chemistry (Clin Chem) Vol. 23 Issue 8 Pg. 1397-401 (Aug 1977) ISSN: 0009-9147 [Print] England
• PMID: 872403 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Hydrazines
• Melanins
• Picrates
• Cysteinyldopa

Topics

• Animals
• Chromatography, Ion Exchange (methods)
• Cricetinae
• Cysteinyldopa (urine)
• Humans
• Hydrazines
• Melanins (urine)
• Melanoma (urine)
• Picrates