Scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on the myocardium from the injury of ischemia-reperfusion.

Abstract

The scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on myocardium from the ischemia-reperfusion injury was studied with electron spin resonance (ESR) and chemiluminescence techniques. Chinonin can effectively inhibit the oxidative activity of ONOO-, (the IC50 = 7 x 10 (-5) mmol/L) and scavenge oxygen free radicals generated from the reaction of xanthine and xanthine oxidase (the IC50 = 2/5 x 10(-4) mmol/l). It is difficult to find another antioxidant which can scavenge so effectively both ONOO- and oxygen free radicals simultaneously. In the system of ischemia-reperfusion injury of myocardium, Chinonin can, in parallel, scavenge the NO and oxygen free radicals generated from the ischemia-reperfused myocardium, and decrease the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart and therefore protect the heart from ischemia-reperfusion injury. The protective effect of 0.1 mmol/l Chinonin is similar to that of 1500 U/ml SOD and catalase.

• Authors: B Zhao, J Shen, M Li, M Li, Q Wan, W Xin
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1315 Issue 2 Pg. 131-7 (Mar 01 1996) ISSN: 0006-3002 [Print] Netherlands
• PMID: 8608170 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Free Radical Scavengers
• Free Radicals
• Glycosides
• Hemoglobins
• Xanthenes
• Xanthones
• mangiferin
• Nitric Oxide
• Arginine
• Catalase
• Nitric Oxide Synthase
• Xanthine Oxidase
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Arginine (analogs & derivatives, pharmacology)
• Catalase (pharmacology)
• Electron Spin Resonance Spectroscopy
• Enzyme Inhibitors (pharmacology)
• Free Radical Scavengers (pharmacology)
• Free Radicals (metabolism)
• Glycosides (pharmacology)
• Heart (drug effects, physiology, physiopathology)
• Hemoglobins (metabolism)
• In Vitro Techniques
• Kinetics
• Luminescent Measurements
• Male
• Myocardial Ischemia (metabolism, prevention & control)
• Myocardial Reperfusion Injury (metabolism, prevention & control)
• Myocardium (metabolism)
• NG-Nitroarginine Methyl Ester
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase (antagonists & inhibitors)
• Oxidation-Reduction
• Rats
• Rats, Sprague-Dawley
• Xanthenes (pharmacology)
• Xanthine Oxidase (metabolism)
• Xanthones