Evidence that the epidermal growth factor receptor on host cells confers reovirus infection efficiency.

Abstract

Reovirus binds to multiple sialoglycoproteins on the host cell surface. In an attempt to probe additional specific determinants that dictate host cell susceptibility to reovirus infection, we found that two mouse cell lines (NR6 and B82) previously shown to express no endogenous epidermal growth factor (EGF) receptors were relatively resistant to reovirus infection, whereas the same cell lines transfected with the gene encoding the EGF receptor manifested significantly higher susceptibility as determined by induction of cytopathic effects, viral protein synthesis, and plaque titration. This enhancement of infection efficiency requires a functional EGF receptor since it was not observed in cells expressing a mutated (kinase-inactive) EGF receptor. The observed difference in infection efficiency is not due to differences in virus binding or internalization. These studies suggest that the reovirus infection process is closely coupled to the EGF receptor-mediated cell signal transduction pathway.

• Authors: J E Strong, D Tang, P W Lee
• Journal: Virology (Virology) Vol. 197 Issue 1 Pg. 405-11 (Nov 1993) ISSN: 0042-6822 [Print] United States
• PMID: 8212574 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Virus
• Sialoglycoproteins
• ErbB Receptors

Topics

• 3T3 Cells
• Animals
• Clone Cells
• ErbB Receptors (genetics, metabolism)
• Humans
• Kinetics
• Mice
• Mutagenesis, Site-Directed
• Receptors, Virus (genetics, metabolism)
• Reoviridae (physiology)
• Sialoglycoproteins (metabolism)
• Signal Transduction
• Time Factors
• Transfection
• Virus Replication