Abstract |
The monoclonal antibody Alz-50 is directed against Alzheimer's disease-related modified tau proteins and reveals cytoskeletal changes, i.e. neurofibrillary tangles and dystrophic neurites. The present study shows that, in the hypothalamus of non-demented control subjects, this same antibody gives a distinctive staining pattern of a subpopulation of somatostatin neurons and beaded fibres. Furthermore, Alz-50 occasionally recognizes somatostatin-containing cell bodies and dystrophic neurite-like fibers in the (neuritic) senile plaques of AD patients. These observations have direct consequences for the interpretation of Alz-50 staining in diagnostic usage and for the assessment of Alzheimer's disease-like changes induced by beta-amyloid in experimental animal brains. On dot spotting, Alz-50 was found to bind to a number of fragments from the somatostatin precursor, of which somatostatin 15-28 stained best. Preadsorption of Alz-50 by somatostatin 15-28, as well as other specificity tests, failed, however, to provide any clue to the nature of the unknown compound(s) stained in the control hypothalamus.
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Authors | J A van de Nes, A A Sluiter, C W Pool, W Kamphorst, R Ravid, D F Swaab |
Journal | Brain research
(Brain Res)
Vol. 655
Issue 1-2
Pg. 97-109
(Aug 29 1994)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 7812796
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alzheimer's disease antigen
- Antigens
- Nerve Tissue Proteins
- Somatostatin
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Alzheimer Disease
(pathology)
- Amino Acid Sequence
- Antibody Specificity
- Antigens
- Cytoskeleton
(physiology)
- Female
- Humans
- Hypothalamus
(cytology, drug effects)
- Immunoblotting
- Immunohistochemistry
- Male
- Middle Aged
- Molecular Sequence Data
- Nerve Tissue Proteins
- Neurons
(drug effects)
- Somatostatin
(physiology)
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